BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0530300 Os05g0530300|J065192E07
         (65 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G14830.1  | chr3:4983386-4985666 FORWARD LENGTH=477             81   1e-16
AT1G53450.1  | chr1:19951747-19953839 REVERSE LENGTH=454           72   4e-14

>AT3G14830.1 | chr3:4983386-4985666 FORWARD LENGTH=477
          Length = 476

 Score = 80.9 bits (198), Expect = 1e-16,   Method: Composition-based stats.
 Identities = 35/50 (70%), Positives = 41/50 (82%)

Query: 7   SFMDVQFPNGQLTYVAGEGITASGFFPFFGGLLQAHGKCPGETRLSFSFK 56
           SFMD+QFPNGQLTYV+GEG+T S F PF GGLLQA G+ PG+ R S+S K
Sbjct: 301 SFMDLQFPNGQLTYVSGEGLTTSAFVPFCGGLLQAQGQYPGDMRFSYSCK 350



>AT1G53450.1 | chr1:19951747-19953839 REVERSE LENGTH=454
          Length = 453

 Score = 72.4 bits (176), Expect = 4e-14,   Method: Compositional matrix adjust.
 Identities = 36/56 (64%), Positives = 42/56 (75%)

Query: 1   LGYLFQSFMDVQFPNGQLTYVAGEGITASGFFPFFGGLLQAHGKCPGETRLSFSFK 56
           L  L  SF+D+QFPNGQLTYV+GEG+T S F P  GGLLQA G+ PG+ R SFS K
Sbjct: 272 LNPLDCSFVDLQFPNGQLTYVSGEGLTTSVFVPLCGGLLQAQGQYPGDMRFSFSCK 327



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.328    0.146    0.455 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 1,405,791
Number of extensions: 45641
Number of successful extensions: 101
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 101
Number of HSP's successfully gapped: 2
Length of query: 65
Length of database: 11,106,569
Length adjustment: 38
Effective length of query: 27
Effective length of database: 10,064,761
Effective search space: 271748547
Effective search space used: 271748547
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 104 (44.7 bits)