BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0527300 Os05g0527300|AK069268
         (363 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G48050.1  | chr5:19472661-19473770 REVERSE LENGTH=370           50   3e-06

>AT5G48050.1 | chr5:19472661-19473770 REVERSE LENGTH=370
          Length = 369

 Score = 49.7 bits (117), Expect = 3e-06,   Method: Compositional matrix adjust.
 Identities = 30/100 (30%), Positives = 50/100 (50%), Gaps = 1/100 (1%)

Query: 113 WQMLEEMYSSVTRARFINTRIALSNTKKGTLSINEYVSKMKALADEMTAAGKIVDDDDLI 172
           W  LE ++     AR +     L  T    LS++EY  K+K+L+D +T     + D  L+
Sbjct: 101 WLSLENLFRDNKEARALQFENELRTTTIDDLSVHEYCQKLKSLSDLLTNVDSPISDRVLV 160

Query: 173 SYIIAGLDDTYEPVISTIVGKDTM-TLGEAYSQLLSFEQR 211
            +++ GL + Y+ +++ I  K    +  EA S LL  E R
Sbjct: 161 MHLLNGLTEKYDYILNVIKHKSPFPSFTEARSMLLMEESR 200



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.313    0.129    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 6,331,517
Number of extensions: 227482
Number of successful extensions: 448
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 448
Number of HSP's successfully gapped: 1
Length of query: 363
Length of database: 11,106,569
Length adjustment: 100
Effective length of query: 263
Effective length of database: 8,364,969
Effective search space: 2199986847
Effective search space used: 2199986847
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 112 (47.8 bits)