BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0521500 Os05g0521500|AK066030
         (554 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G25740.1  | chr2:10980175-10983632 FORWARD LENGTH=548          472   e-133

>AT2G25740.1 | chr2:10980175-10983632 FORWARD LENGTH=548
          Length = 547

 Score =  472 bits (1214), Expect = e-133,   Method: Compositional matrix adjust.
 Identities = 257/567 (45%), Positives = 352/567 (62%), Gaps = 60/567 (10%)

Query: 10  RERRQMEEILQLDMEELNVEMVNDDGSSSEEANGGDDGADAFLREGVASTS---GQFTFN 66
           RER Q+E+I +LD EEL VE V+D   S  + N  D  +  F     AS +    +  FN
Sbjct: 9   RERLQIEQIRELDFEELQVEEVDDLHDSDSDDNNDDLSSFPFSSHAQASGNLGDDELMFN 68

Query: 67  TSLASLHTYLGEVDDTRGRVSLLDGGTILSLPMLYLQGVVLFPGATLPLRLIQGRFVAAV 126
            +LASLH YLGEV+DT+ RVS +DGGT+L +P+ YL+GVVLFP ATLPLR+IQ  F+AAV
Sbjct: 69  PALASLHMYLGEVEDTQNRVSFVDGGTVLKIPLFYLEGVVLFPEATLPLRIIQPSFLAAV 128

Query: 127 EKALRQVDAPCTIGVVLMYKRHSTRHYANASVGTTAEIRQLGRMDDGSLNVVARGQQRFR 186
           E+AL Q +AP TIGV+ +Y+  +   YA  SVGTTAEIRQ  R+ DGS NV+ RGQQRFR
Sbjct: 129 ERALNQANAPSTIGVIRVYREGAQFKYA--SVGTTAEIRQYRRLGDGSFNVITRGQQRFR 186

Query: 187 LKRHWMXXXXXXXXXXQIIEEDTPLRTPRDAFAQLASCNSLRQHTSSPVISLDVSPIKQR 246
           LK  W           QI++ED PLRTPRDAF +L   + LR        SL  +P++  
Sbjct: 187 LKHRWTDVEGFTCGEVQIVDEDVPLRTPRDAFGKLVPLSKLRGRYPLGTASLS-TPLRDM 245

Query: 247 D---HVDSELDCGTP-SPKSTASNHSAIXXXXXXXXXXXXXXXXXXNEDGIFMHEQFYSQ 302
           D     +SE    +  SP     ++S +                    D IF +    S 
Sbjct: 246 DAQSEANSEESFESALSPSEKRLHYSVV--------------------DSIFCNST--SS 283

Query: 303 ELHPLKGSAAVQSGENTNMGEEDFCLTSLRSLSSAGTRDTKEQRQYI----LPKQHFQ-- 356
           +   +  ++ VQS      G   + L S+  L+S+   + ++++  I    + ++ +Q  
Sbjct: 284 DDDQVVSTSTVQSS-----GSNPYSLRSIGCLASSHDNENEDEQSAIGKTPVSQEKYQKQ 338

Query: 357 -----------------APLSFWPRWAYQMYDSYALARRAADLWRQIITHPSMDDYVRKP 399
                             P +FWP WAY+M+DSY LA+RA DLW+QI+  P+M+ +V KP
Sbjct: 339 NRLASFRQNTDLSRFRMTPRAFWPFWAYRMFDSYYLAQRAVDLWKQIVGVPNMEAFVNKP 398

Query: 400 DLLSFSIGSKLPVSESVRQKLLEIDGISYRLRREIQLLKAFNLIKCRCCKSLIAKRSDMV 459
           D+LSFSI SK+PVSES+RQ+LLE+DG+SYRL+REI+LL++F+ ++C  C+++IA+R DM+
Sbjct: 399 DILSFSIASKIPVSESIRQELLELDGVSYRLQREIELLESFDRVRCIHCQTVIARRKDML 458

Query: 460 VMSTDGPLGAYVNPHGFVHETITVSRATGLALDGNPSTVHSWFPGYSWTIASCTDCESNI 519
           VMS +GPLGAYVNPHG+VHE +T  +A  +AL G P    SWFPGY+WTIA+C  CE+ +
Sbjct: 459 VMSNEGPLGAYVNPHGYVHEIMTFYKANDIALRGRPVKKDSWFPGYAWTIANCATCETQL 518

Query: 520 GWLFKATKKNLRPRSFWGIRSSQIADD 546
           GW F AT K L+P SFW +R SQ+ADD
Sbjct: 519 GWHFTATNKKLKPSSFWAVRGSQVADD 545



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.318    0.133    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 11,452,674
Number of extensions: 465026
Number of successful extensions: 1151
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 1152
Number of HSP's successfully gapped: 2
Length of query: 554
Length of database: 11,106,569
Length adjustment: 104
Effective length of query: 450
Effective length of database: 8,255,305
Effective search space: 3714887250
Effective search space used: 3714887250
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 114 (48.5 bits)