BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0515700 Os05g0515700|AK069076
         (641 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G28520.1  | chr1:10029713-10031479 FORWARD LENGTH=487          339   2e-93
AT2G42400.1  | chr2:17654490-17656281 REVERSE LENGTH=451          291   6e-79

>AT1G28520.1 | chr1:10029713-10031479 FORWARD LENGTH=487
          Length = 486

 Score =  339 bits (870), Expect = 2e-93,   Method: Compositional matrix adjust.
 Identities = 172/305 (56%), Positives = 209/305 (68%), Gaps = 14/305 (4%)

Query: 258 NTMQGIGQLE-HQQYNLPLDLPPNSFVDANNSAQSSGDVF--FHMSDLLTTMCPSPSQYL 314
           N   G   LE HQQY+L  +  PN F    N+  S G V    H+S+ + T+CP PS +L
Sbjct: 155 NNFDGTAPLEYHQQYDLQQEFEPN-FNGGFNNCPSYGVVEGPIHISNFIPTICPPPSAFL 213

Query: 315 GPKCALWDCGRPVRGSDECQHYCNPYHXXXXXXXXXXXXTRPVMRPRGIDLKDGPLFAAL 374
           GPKCALWDC RP +G D  Q YC+ +H              PV+RP GI LKDG LFAAL
Sbjct: 214 GPKCALWDCPRPAQGFDWFQDYCSSFHAALAFNEGPPGMN-PVVRPGGIGLKDGLLFAAL 272

Query: 375 SAKVQGKNVGIPVCEGAATTKSPWNAPELFDLSLLEGESLREWLFFDTPRRAFDSGNRKQ 434
           SAK  GK+VGIP CEGAAT KSPWNAPELFDL++LE E+LREWLFFD PRRAF+SGNRKQ
Sbjct: 273 SAKAGGKDVGIPECEGAATAKSPWNAPELFDLTVLESETLREWLFFDKPRRAFESGNRKQ 332

Query: 435 RSLPDYNGRGWHESRKQVMKDFGGLKRSYYMDPQPSSNYEWHLFEYETNDSDALALYRLE 494
           RSLPDYNGRGWHESRKQ+M +FGGLKRSYYMDPQP  ++EWHL+EYE N  DA ALYRLE
Sbjct: 333 RSLPDYNGRGWHESRKQIMVEFGGLKRSYYMDPQPLHHFEWHLYEYEINKCDACALYRLE 392

Query: 495 YKSSDTKRSVKSKLASSPLSEIQQQMVRLSADSPVE---------SKRTARSRAKANQKD 545
            K  D K++ K K+++  ++++Q+QM RL+A+ P E         +KR  + R K + K 
Sbjct: 393 LKLVDGKKTSKGKVSNDSVADLQKQMGRLTAEFPPENNTTNTTNNNKRCIKGRPKVSTKV 452

Query: 546 NNSNA 550
              N 
Sbjct: 453 ATGNV 457




 Score = 98.6 bits (244), Expect = 9e-21,   Method: Compositional matrix adjust.
 Identities = 61/136 (44%), Positives = 76/136 (55%), Gaps = 9/136 (6%)

Query: 28  HQQFRNLAKTRVDDLQEMFSGLQSARKESRSADAALLEEQVHHMLREWRAELNVPSPAXX 87
           H+ F++ AK RVDDLQ M   LQ ARKESR  D  LLEEQV+ MLREW++ELN PSPA  
Sbjct: 15  HKLFKDKAKNRVDDLQGMLLDLQFARKESRPTDVTLLEEQVNQMLREWKSELNEPSPASS 74

Query: 88  XXXXXXXXXXREASDPPSETLRLLQLAGAEEEDDATSKLVMPRSPMPMQSSHEGHNLSPV 147
                            S+  RLLQL   +EEDDATSKL  P+ P P   + E    +  
Sbjct: 75  LQQGGTLGSFS------SDICRLLQL--CDEEDDATSKLAAPK-PEPADQNLEAGKAAVF 125

Query: 148 LQGGTMAGGAAELMVP 163
            +G  +  G +E  +P
Sbjct: 126 QRGYNLVQGKSEHGLP 141



>AT2G42400.1 | chr2:17654490-17656281 REVERSE LENGTH=451
          Length = 450

 Score =  291 bits (746), Expect = 6e-79,   Method: Compositional matrix adjust.
 Identities = 145/256 (56%), Positives = 179/256 (69%), Gaps = 8/256 (3%)

Query: 275 LDLPPNSFVDANNSAQSSGDVFFHMSDLLTTMCPSPSQYLGPKCALWDCGRPVRGSDECQ 334
           LD  P++ +D++       D F   S   +   P PS +LGPKCALWDC RP +GS+   
Sbjct: 192 LDNSPSTKLDSSEEI----DNFAEFSTPSSVRVP-PSAFLGPKCALWDCTRPAQGSEWYL 246

Query: 335 HYCNPYHXXXXXXXXXXXXTRPVMRPRGIDLKDGPLFAALSAKVQGKNVGIPVCEGAATT 394
            YC+ YH            T PV+RP GI LKD  L  AL AK QGKNVGIPVCEGA  T
Sbjct: 247 DYCSNYHGTLALNEDSPG-TAPVLRPGGISLKDNLLIDALRAKTQGKNVGIPVCEGAVNT 305

Query: 395 KSPWNAPELFDLSLLEGESLREWLFFDTPRRAFDSGNRKQRSLPDYNGRGWHESRKQVMK 454
           K PWNA ELF L L+EGE++REWLFFD PRRA+DSGNRKQRSLPDY+GRGWHESRKQ+MK
Sbjct: 306 KCPWNAAELFHLELVEGETIREWLFFDKPRRAYDSGNRKQRSLPDYSGRGWHESRKQLMK 365

Query: 455 DFGGLKRSYYMDPQPSSNYEWHLFEYETNDSDALALYRLEYKSSDTKRSVKSKLASSPLS 514
           +  G KRSYYMDPQP   +EWHLFEY+ N+SDA ALYRLE K  + K+S K K++  PL+
Sbjct: 366 EQEGQKRSYYMDPQPPGPFEWHLFEYQINESDACALYRLELKVGNGKKSPKGKISKDPLA 425

Query: 515 EIQQQM--VRLSADSP 528
           ++Q++M   ++++D P
Sbjct: 426 DLQKKMGQFKVASDKP 441




 Score = 60.5 bits (145), Expect = 2e-09,   Method: Compositional matrix adjust.
 Identities = 38/92 (41%), Positives = 49/92 (53%), Gaps = 10/92 (10%)

Query: 35  AKTRVDDLQEMFSGLQSARKESRSADAALLEEQVHHMLREWRAELNVPSPAXXXXXXXXX 94
            + ++ +LQE F  LQ+ARKE R  D ALLE Q+   +REW+AEL  PSP          
Sbjct: 14  VEEKLRELQERFCHLQAARKEGRHGDLALLEAQISQNIREWQAELTAPSPESSLLGEGIS 73

Query: 95  XXXREASDPPSETLRLLQLAGAEEEDDATSKL 126
               E +        LL+L   +EEDDATS L
Sbjct: 74  QFLEEFAP-------LLKL---DEEDDATSTL 95



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.313    0.129    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 13,238,860
Number of extensions: 562661
Number of successful extensions: 1173
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 1169
Number of HSP's successfully gapped: 4
Length of query: 641
Length of database: 11,106,569
Length adjustment: 105
Effective length of query: 536
Effective length of database: 8,227,889
Effective search space: 4410148504
Effective search space used: 4410148504
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 115 (48.9 bits)