BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0490500 Os05g0490500|AK066923
         (216 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G32760.1  | chr2:13892140-13894295 FORWARD LENGTH=353          133   8e-32

>AT2G32760.1 | chr2:13892140-13894295 FORWARD LENGTH=353
          Length = 352

 Score =  133 bits (334), Expect = 8e-32,   Method: Compositional matrix adjust.
 Identities = 82/215 (38%), Positives = 124/215 (57%), Gaps = 23/215 (10%)

Query: 2   RQQSMIGQVAALYPVKVFHDLPHGRNLNSNTNGAHRSLSEENGTLPEENGTHLLNVIKLP 61
           RQQ M+ QV+ +YP+K+       + L S   G+        GT P   G+  + ++ LP
Sbjct: 156 RQQFMVSQVSFIYPLKIEAGPSQDQELESFPGGSRL------GTKPLSQGS--VRILGLP 207

Query: 62  -QIHALTFLGWQIGKQRRKQNDICEKDLQRSAAVLGYAAHAILLIASYLHVPLRYPLHFG 120
             +   T + +   K          K++Q+SA  LGY AHA+ LIA YL VP+RYPL  G
Sbjct: 208 FSMAPFTKMSFFTDK----------KEVQKSATALGYVAHAVSLIAPYLRVPIRYPLCLG 257

Query: 121 GSRSYVSDCLPSAETASIASAERTCINMTDPKLTVYPLFVECQEDDPTKASYAIYLLHKD 180
           GS++Y+ D  P  E +    +  T ++  +     +PLF++ Q  D T+A+YA++LL+K+
Sbjct: 258 GSKTYIRDYAPYIEPSPSDMSPITTLSQ-NINFVEFPLFLDGQ--DTTRAAYAVFLLNKN 314

Query: 181 TEQLLNYIGAESSG-RRVFDNLQELIRIIQSDEYV 214
            EQLLN++G  S G R+V  NL+ELIRIIQS +Y+
Sbjct: 315 IEQLLNFVGENSLGPRQVLANLKELIRIIQSPDYI 349



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.319    0.134    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,835,579
Number of extensions: 202649
Number of successful extensions: 434
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 431
Number of HSP's successfully gapped: 1
Length of query: 216
Length of database: 11,106,569
Length adjustment: 94
Effective length of query: 122
Effective length of database: 8,529,465
Effective search space: 1040594730
Effective search space used: 1040594730
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 109 (46.6 bits)