BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0472300 Os05g0472300|AK071966
         (316 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G43190.1  | chr2:17956220-17957833 FORWARD LENGTH=297          196   2e-50

>AT2G43190.1 | chr2:17956220-17957833 FORWARD LENGTH=297
          Length = 296

 Score =  196 bits (497), Expect = 2e-50,   Method: Compositional matrix adjust.
 Identities = 105/215 (48%), Positives = 146/215 (67%), Gaps = 3/215 (1%)

Query: 89  YSELSFAPHDNLLQRSISDLDSMD-VVHNVVYDIIQKGGDAGKITKGAKKLKLEKGILLD 147
           Y++LS    +NLL  ++    +   +V  V++++++ G  A K  +G K +KL+  ILLD
Sbjct: 81  YTKLSHPVDENLLATNVKFSSAKGTIVDKVLHNLLRSGDSAQKYLQGTKSVKLDNYILLD 140

Query: 148 NYVHRGPRLVDAQAKSLLIHSKRSKRHMSLKQHKKCGSFDLPETLRKFDLYKPMHEMWKE 207
           N+V +         K+    SKRSK  MS+K+ KK G+  +P+ L+KFDL+KPMH MW+ 
Sbjct: 141 NFV-QSRSSASGSKKASQKDSKRSKSRMSMKRLKKSGALHIPKDLQKFDLFKPMHGMWES 199

Query: 208 YITELTKSTPKKQLSETLLSADLHGALVIVAECKSASSYQGVCGIMIRDTAETFGIISDD 267
           Y+ +L K T K QLS TLLSADLHGA + VAECK A S+ GV GIM+R+T+ETFGII+ D
Sbjct: 200 YMMKLIKVTGKIQLSLTLLSADLHGAFMFVAECKIA-SFTGVQGIMVRETSETFGIITRD 258

Query: 268 NRFRVVPKAGSVFILQADCWKVTLIGDKLSPREKL 302
           ++FRVVPK  SVFI+Q DCWK+TL GDK   R+ +
Sbjct: 259 DKFRVVPKKLSVFIIQLDCWKITLHGDKFISRDNI 293



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.316    0.131    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 6,359,382
Number of extensions: 250021
Number of successful extensions: 693
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 691
Number of HSP's successfully gapped: 1
Length of query: 316
Length of database: 11,106,569
Length adjustment: 99
Effective length of query: 217
Effective length of database: 8,392,385
Effective search space: 1821147545
Effective search space used: 1821147545
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 112 (47.8 bits)