BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0462400 Os05g0462400|AK099608
         (884 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G09560.2  | chr3:2934953-2938673 REVERSE LENGTH=905            216   4e-56
AT5G42870.1  | chr5:17185719-17189681 REVERSE LENGTH=931          158   1e-38

>AT3G09560.2 | chr3:2934953-2938673 REVERSE LENGTH=905
          Length = 904

 Score =  216 bits (550), Expect = 4e-56,   Method: Compositional matrix adjust.
 Identities = 111/225 (49%), Positives = 152/225 (67%), Gaps = 8/225 (3%)

Query: 667 FEISLCGHMLRPGMGQASAEEVFQQHLVLEEDFKLSGSSIMKNVNLVVKFDSQYFLWSKI 726
           +E+SLC   LR GMG ++A EVF  H++ +E++  S +SI+++ NLVV+    Y  W+K 
Sbjct: 491 YELSLCKDELRQGMGLSAAAEVFDAHMISKEEYINSATSILESENLVVRIRETYMPWTKA 550

Query: 727 SHIILGKAVFGPNFCTEHIDAIPVEHQETPTSRD---SLGLSPSSRRWRLWNIPFRIXXX 783
           + I+LGKAVF  +   +  D I VE  E+P  +D   ++  S S  RWRLW IPFR    
Sbjct: 551 ARIVLGKAVFDLDLDIQPDDVISVEENESPKPKDDETTITPSSSGTRWRLWPIPFRRVKT 610

Query: 784 X--XXXXXXXXEDIFLDTETVL--SPMDEQTPENNINQSPRKQFVRTLIPTSEQVASLNL 839
                      ED+F+D+E  L  SP  + T E+  ++SPR+Q VRT +PT+EQ+ASLNL
Sbjct: 611 VEHTGSNSSSEEDLFVDSEPGLQNSPETQSTTESR-HESPRRQLVRTNVPTNEQIASLNL 669

Query: 840 KEGQNIVTFSFSTRVLGKQQVEAHIYLWKWNAKIVISDVDGTITR 884
           K+GQN++TFSFSTRVLG QQV+AHIY W+W+ KIVISDVDGTIT+
Sbjct: 670 KDGQNMITFSFSTRVLGTQQVDAHIYRWRWDTKIVISDVDGTITK 714




 Score =  206 bits (523), Expect = 6e-53,   Method: Compositional matrix adjust.
 Identities = 114/247 (46%), Positives = 146/247 (59%), Gaps = 21/247 (8%)

Query: 1   MNVVGRVGSLISQGVYSVATPFHPFGGAVDIIAVEQPDGSYRSTPWYVRFGKFQGVLKGA 60
           M++VGRVGSLISQGVYSVATPFHPFGGA+D+I V+Q DGS+RSTPWYVRFGKFQGVLKGA
Sbjct: 1   MSLVGRVGSLISQGVYSVATPFHPFGGAIDVIVVQQQDGSFRSTPWYVRFGKFQGVLKGA 60

Query: 61  EKVVTITVNGVDASFHMQLDNSGQAYFMRELVXXXXXXXX----XXXXXXXXXXXXXXXX 116
           EK V I+VNG +A FHM LDNSG+AYF+RE+                             
Sbjct: 61  EKFVRISVNGTEADFHMYLDNSGEAYFIREVDPAANDTNNLISGSENNNGNQNNGVTYRL 120

Query: 117 XKSDGDLYIGAKGRPGSPESNVECQEKQAIEEF-----------DSYGYSRME-VAEDSA 164
             S  D   G       P S +E  E      F             YG +R + +  +S 
Sbjct: 121 EHSLSDSGTGELREGFDPLSRLERTESDCNRRFYDFQDDPPSPTSEYGSARFDNLNVESY 180

Query: 165 KQPDGSNSEMVLVSVDGHVLTAPISSTEENVEDVQLSEPQFHLGPGESS-----SGDFSR 219
               GS+SE+VLVS+DGH+LTAP+S  E+  E+++L+ PQFHL PG+ +     + +F+ 
Sbjct: 181 GDSQGSDSEVVLVSIDGHILTAPVSVAEQEAENLRLNTPQFHLAPGDGTEFCEGNTEFAS 240

Query: 220 SGEVWEA 226
           S   W+ 
Sbjct: 241 SETPWDT 247



>AT5G42870.1 | chr5:17185719-17189681 REVERSE LENGTH=931
          Length = 930

 Score =  158 bits (399), Expect = 1e-38,   Method: Compositional matrix adjust.
 Identities = 95/221 (42%), Positives = 119/221 (53%), Gaps = 24/221 (10%)

Query: 668 EISLCGHMLRPGMGQASAEEVFQQHLVLEEDFKLSGSSIMKNVNLVVKFDSQYFLWSKIS 727
           E+SLC H+L  GMG  +A + F    +  E F   G SI++N  LVVK    YF W   +
Sbjct: 541 ELSLCKHLLSEGMGAEAASQAFNSEKLDMEKFASLGPSILENDKLVVKIGGCYFPWDAAA 600

Query: 728 HIILGKAVFGPNFCTEHIDAIPVEHQETPTSRDSLGLSPSSRRWRLWNIPFRIXXXXXXX 787
            IILG   FG     E    I V+  E P       L+  S  W+LW  PF +       
Sbjct: 601 PIILGVVSFGTAQVFEPKGMIAVDRNEKPGDV----LAQGSGSWKLW--PFSLRRSTK-- 652

Query: 788 XXXXXEDIFLDTETVLSPM-DEQTPENNINQS---PRKQFVRTLIPTSEQVASLNLKEGQ 843
                       E   SP  D   PE    +S   P K+ VR L PTSEQ+ASL+LK+G 
Sbjct: 653 ------------EAEASPSGDTAEPEEKQEKSSPRPMKKTVRALTPTSEQLASLDLKDGM 700

Query: 844 NIVTFSFSTRVLGKQQVEAHIYLWKWNAKIVISDVDGTITR 884
           N VTF+FST ++G QQV+A IYLWKWN++IV+SDVDGTITR
Sbjct: 701 NSVTFTFSTNIVGTQQVDARIYLWKWNSRIVVSDVDGTITR 741




 Score =  141 bits (356), Expect = 1e-33,   Method: Compositional matrix adjust.
 Identities = 59/91 (64%), Positives = 76/91 (83%)

Query: 1  MNVVGRVGSLISQGVYSVATPFHPFGGAVDIIAVEQPDGSYRSTPWYVRFGKFQGVLKGA 60
          MN VGR+GS I +GV +V+ PFHPFGGA+DII VEQPDG+++S+PWYVRFGKFQGVLK  
Sbjct: 1  MNAVGRIGSYIYRGVGTVSGPFHPFGGAIDIIVVEQPDGTFKSSPWYVRFGKFQGVLKNG 60

Query: 61 EKVVTITVNGVDASFHMQLDNSGQAYFMREL 91
            ++ I VNGVD+ F+M L ++GQAYF+RE+
Sbjct: 61 RNLIRIDVNGVDSGFNMYLAHTGQAYFLREV 91



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.310    0.130    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 20,451,501
Number of extensions: 949106
Number of successful extensions: 2080
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 2077
Number of HSP's successfully gapped: 5
Length of query: 884
Length of database: 11,106,569
Length adjustment: 107
Effective length of query: 777
Effective length of database: 8,173,057
Effective search space: 6350465289
Effective search space used: 6350465289
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 116 (49.3 bits)