BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os05g0462000 Os05g0462000|Os05g0462000
(102 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT5G02580.1 | chr5:579965-580454 FORWARD LENGTH=92 94 2e-20
AT3G55240.1 | chr3:20473876-20474705 REVERSE LENGTH=96 86 5e-18
AT3G28990.1 | chr3:11002351-11003046 REVERSE LENGTH=89 76 4e-15
AT1G10657.1 | chr1:3530652-3531522 FORWARD LENGTH=126 56 4e-09
>AT5G02580.1 | chr5:579965-580454 FORWARD LENGTH=92
Length = 91
Score = 93.6 bits (231), Expect = 2e-20, Method: Compositional matrix adjust.
Identities = 46/86 (53%), Positives = 57/86 (66%), Gaps = 1/86 (1%)
Query: 1 MGD-SSSSASYIRMVHHLIEKCICFNLNKEECMEALEKHANINPVVTSTVWXXXXXXXXX 59
MGD +SS ASYI +VHHLIE+CI FN+ KEECM+AL KHANI P++TSTVW
Sbjct: 1 MGDHNSSQASYIHLVHHLIEECIVFNMGKEECMDALFKHANIKPIITSTVWKELAKENKE 60
Query: 60 XXXTYNKDRAERNIEAETMQRIQKML 85
Y + R E E ET +RI+ +L
Sbjct: 61 FFEAYERRREEIPTEKETARRIRDLL 86
>AT3G55240.1 | chr3:20473876-20474705 REVERSE LENGTH=96
Length = 95
Score = 85.5 bits (210), Expect = 5e-18, Method: Compositional matrix adjust.
Identities = 45/87 (51%), Positives = 57/87 (65%), Gaps = 3/87 (3%)
Query: 1 MGDSSSSASYIRMVHHLIEKCICFNLNKEECMEALEKHANINPVVTSTVWXXXXXXXXXX 60
M DSSS ASYI MV H+IEKC+ F+++KEEC+EAL KHANI PV+TSTVW
Sbjct: 1 MADSSS-ASYIHMVQHMIEKCLIFHMSKEECVEALSKHANITPVITSTVWKELEKENKEF 59
Query: 61 XXTYN--KDRAERNIEAETMQRIQKML 85
Y + + E+ E ET Q IQK++
Sbjct: 60 FKAYEERQSKQEQMSEEETNQMIQKII 86
>AT3G28990.1 | chr3:11002351-11003046 REVERSE LENGTH=89
Length = 88
Score = 75.9 bits (185), Expect = 4e-15, Method: Compositional matrix adjust.
Identities = 39/83 (46%), Positives = 52/83 (62%), Gaps = 2/83 (2%)
Query: 5 SSSASYIRMVHHLIEKCICFNLNKEECMEALEKHANINPVVTSTVWXXXXXXXXXXXXTY 64
SS A YI +V H+IE C+ FN++KEECMEAL ++ANINP++TSTVW TY
Sbjct: 4 SSPAVYIHLVQHMIETCLTFNMSKEECMEALSENANINPIITSTVWKELVKENKDFFETY 63
Query: 65 NKD--RAERNIEAETMQRIQKML 85
+ + E E ET Q IQ ++
Sbjct: 64 EQKLMKKESMSEEETNQLIQNII 86
>AT1G10657.1 | chr1:3530652-3531522 FORWARD LENGTH=126
Length = 125
Score = 55.8 bits (133), Expect = 4e-09, Method: Compositional matrix adjust.
Identities = 23/42 (54%), Positives = 34/42 (80%)
Query: 9 SYIRMVHHLIEKCICFNLNKEECMEALEKHANINPVVTSTVW 50
SYIRMV H+IE+CI + ++EC++AL+ HA+I P+VT TV+
Sbjct: 22 SYIRMVQHMIERCILLRMTRDECVKALDHHASILPLVTLTVF 63
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.316 0.125 0.362
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 1,425,516
Number of extensions: 38347
Number of successful extensions: 78
Number of sequences better than 1.0e-05: 4
Number of HSP's gapped: 78
Number of HSP's successfully gapped: 4
Length of query: 102
Length of database: 11,106,569
Length adjustment: 72
Effective length of query: 30
Effective length of database: 9,132,617
Effective search space: 273978510
Effective search space used: 273978510
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 104 (44.7 bits)