BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os05g0399100 Os05g0399100|AK073623
(284 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT3G23600.1 | chr3:8473833-8475655 FORWARD LENGTH=240 293 6e-80
AT3G23570.1 | chr3:8458052-8459608 REVERSE LENGTH=240 273 8e-74
AT1G35420.2 | chr1:13026320-13027463 FORWARD LENGTH=316 79 2e-15
>AT3G23600.1 | chr3:8473833-8475655 FORWARD LENGTH=240
Length = 239
Score = 293 bits (751), Expect = 6e-80, Method: Compositional matrix adjust.
Identities = 140/240 (58%), Positives = 173/240 (72%), Gaps = 1/240 (0%)
Query: 45 MASPQCCANPPTLNPAGGEGKVVESFGGIKAYVAGAAESKAAVVLISDVFGFEAPNLRKI 104
M+ PQCC NPPTLNP G G V E GG+ AYV+G+AESK V+LISD+FGFEAPNLR +
Sbjct: 1 MSGPQCCENPPTLNPVSGSGHV-EKLGGLDAYVSGSAESKLCVLLISDIFGFEAPNLRAL 59
Query: 105 ADKVASSGYFVVVPDFLHGDPLVPESTEKPFQMWIKEHGPDKAFEEAKPIIAALKEKGVS 164
ADKVA+SG++VVVPD+ GDP P + ++P +WIK+HG DK FE KP++ +K KG++
Sbjct: 60 ADKVAASGFYVVVPDYFGGDPYNPSNQDRPIPVWIKDHGCDKGFENTKPVLETIKNKGIT 119
Query: 165 SIGAVGYCWGAKVVVELVKAHEIQAAVMCHPSFVTVDDMKEVKCPIAILGAEIDRMSPPE 224
+IGA G CWGAKVVVEL K IQAAV+ HPSFV VDD+K K PIAILGAEID+MSPP
Sbjct: 120 AIGAAGMCWGAKVVVELSKEELIQAAVLLHPSFVNVDDIKGGKAPIAILGAEIDQMSPPA 179
Query: 225 VVKQFEQVLSSKSGIGHFVKIFPGVEHGWTVRYKNXXXXXXXXXXXXXXXMIDWFNKNLK 284
++KQFE++LSSK + +VKI P V HGWTVRY M+DWF +K
Sbjct: 180 LLKQFEEILSSKPEVNSYVKIHPKVSHGWTVRYNIDEPEAVKAAEEAHKEMLDWFVTYIK 239
>AT3G23570.1 | chr3:8458052-8459608 REVERSE LENGTH=240
Length = 239
Score = 273 bits (698), Expect = 8e-74, Method: Compositional matrix adjust.
Identities = 128/240 (53%), Positives = 165/240 (68%), Gaps = 1/240 (0%)
Query: 45 MASPQCCANPPTLNPAGGEGKVVESFGGIKAYVAGAAESKAAVVLISDVFGFEAPNLRKI 104
M+ QC NPP L+P G G V E G + YV G+ SK AV+L+ VFG+E PNLRK+
Sbjct: 1 MSGHQCTENPPDLDPTSGSGHV-EKLGNLDTYVCGSTHSKLAVLLVPHVFGYETPNLRKL 59
Query: 105 ADKVASSGYFVVVPDFLHGDPLVPESTEKPFQMWIKEHGPDKAFEEAKPIIAALKEKGVS 164
ADKVA +G++ VVPDF HGDP PE+ ++PF +W+K+H +K FEE+KPI+ ALK KG++
Sbjct: 60 ADKVAEAGFYAVVPDFFHGDPYNPENQDRPFPIWMKDHELEKGFEESKPIVEALKNKGIT 119
Query: 165 SIGAVGYCWGAKVVVELVKAHEIQAAVMCHPSFVTVDDMKEVKCPIAILGAEIDRMSPPE 224
SIGA G+CWGAKV VEL K + A V+ HP+ VTVDD+KEV PIA+LGAEID++SPPE
Sbjct: 120 SIGAAGFCWGAKVAVELAKEKLVDATVLLHPARVTVDDIKEVNLPIAVLGAEIDQVSPPE 179
Query: 225 VVKQFEQVLSSKSGIGHFVKIFPGVEHGWTVRYKNXXXXXXXXXXXXXXXMIDWFNKNLK 284
+V+QFE +L+SK + FVKIFP +HGWTVRY M+ W LK
Sbjct: 180 LVRQFEDILASKPQVKSFVKIFPRCKHGWTVRYNENDPSEVEAAMEAHKDMLAWLIDYLK 239
>AT1G35420.2 | chr1:13026320-13027463 FORWARD LENGTH=316
Length = 315
Score = 79.0 bits (193), Expect = 2e-15, Method: Compositional matrix adjust.
Identities = 51/198 (25%), Positives = 96/198 (48%), Gaps = 14/198 (7%)
Query: 69 SFGGIKAYVAGAAESK--AAVVLISDVFGFEAPNLRKIADKVASSGYFVVVPDFLHGDPL 126
S G++ Y+ A ++ ++L+SDVFGF+ R A +VA +GY V+VPD GDP
Sbjct: 96 SVDGVEGYLLTAVKNNNGTGLLLLSDVFGFQDSATRDFAYRVACNGYNVLVPDLFRGDPW 155
Query: 127 VPESTEKPFQMWIKEHGPDKAFEEAKPIIAALKEK----GVS-SIGAVGYCWGAKVVVEL 181
+ ++ W + H P++ ++ + E+ G+S +G +G+C+G VV++
Sbjct: 156 SKNRPKSEYEEWRRGHDPNRIRQDTTTFTKWMVEEFAAAGISKKLGVMGFCFGGGRVVDV 215
Query: 182 VKAHE---IQAAVMCHPSFVTVDDMKEVKCPIAILGAEIDRMSPPEVVKQFEQVLSSKSG 238
+ E V + + + +VK P+ + + D + + + + E+ K G
Sbjct: 216 LATDESGYFSTGVSFYGTRIDSAVAGDVKVPVLFIAGDRDPLCEVKGLYEIEE----KIG 271
Query: 239 IGHFVKIFPGVEHGWTVR 256
G V ++ G HG+ R
Sbjct: 272 EGSKVVVYEGRGHGFVHR 289
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.319 0.135 0.414
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 6,186,372
Number of extensions: 254141
Number of successful extensions: 690
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 689
Number of HSP's successfully gapped: 3
Length of query: 284
Length of database: 11,106,569
Length adjustment: 97
Effective length of query: 187
Effective length of database: 8,447,217
Effective search space: 1579629579
Effective search space used: 1579629579
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 111 (47.4 bits)