BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0376300 Os05g0376300|AK068730
         (326 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT4G31400.1  | chr4:15237411-15239266 FORWARD LENGTH=346          221   3e-58

>AT4G31400.1 | chr4:15237411-15239266 FORWARD LENGTH=346
          Length = 345

 Score =  221 bits (564), Expect = 3e-58,   Method: Compositional matrix adjust.
 Identities = 126/272 (46%), Positives = 161/272 (59%), Gaps = 12/272 (4%)

Query: 55  KKRNYAQFHLELGQPDFLLHMCSVCGMMYARGNDDDEKAHKAYHKSYFEGVPFKGWRNET 114
           KKRNY QFHLELGQ DFLL  C+ CG  YA G++ DEK H+++HK Y  G+PFKGW+NE 
Sbjct: 77  KKRNYTQFHLELGQSDFLLRHCAECGAKYAPGDELDEKNHQSFHKDYMYGLPFKGWQNEK 136

Query: 115 VVARSEG-GDRIILVTDENSCARNSXXXXXXXXXXXXLGFGEGQLLHKLCKVYLFISSQR 173
                    +RI++V++ +S A  +            L  GE  +LH+ CKVYLFISSQR
Sbjct: 137 AFTSPLFIKNRIVMVSENDSPAHRNKVQEVVKMMEVEL--GEDWILHQHCKVYLFISSQR 194

Query: 174 IVGCLVVEPIKTAHKVIPGSTEENGSDLPVDKIEPVKTNHTLAFGKISFKREVLKRHDHS 233
           I GCLV EPIK A K+I    +E      + K      + ++ FG I  +REV KR   S
Sbjct: 195 ISGCLVAEPIKEAFKLIASPDDER----QLQKESSSSPSTSIQFGNIVLQREVSKRCRTS 250

Query: 234 DKNKEEYRDPGAIICEKEDVPAHCGFRAIWVVPSRRRKRIGSQLMDAARKSFL-EGETLC 292
           D    +  D G I+CE+E  PA CG RAIWV PS RRK I + L+D  R+SF   G  L 
Sbjct: 251 D----DRLDNGVIVCEEEAKPAVCGIRAIWVSPSNRRKGIATWLLDTTRESFCNNGCMLE 306

Query: 293 ISQCAFSPPTSSGKALARSYCKTSAFLVYKEQ 324
            SQ AFS P+S G++    Y  T +FL+YK Q
Sbjct: 307 KSQLAFSQPSSIGRSFGSKYFGTCSFLLYKAQ 338



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.318    0.135    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 7,738,660
Number of extensions: 335688
Number of successful extensions: 689
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 685
Number of HSP's successfully gapped: 1
Length of query: 326
Length of database: 11,106,569
Length adjustment: 99
Effective length of query: 227
Effective length of database: 8,392,385
Effective search space: 1905071395
Effective search space used: 1905071395
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 112 (47.8 bits)