BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0290300 Os05g0290300|J065165N22
         (185 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G57170.1  | chr3:21159620-21162259 REVERSE LENGTH=561          161   1e-40

>AT3G57170.1 | chr3:21159620-21162259 REVERSE LENGTH=561
          Length = 560

 Score =  161 bits (408), Expect = 1e-40,   Method: Compositional matrix adjust.
 Identities = 82/161 (50%), Positives = 106/161 (65%)

Query: 25  VEYAHKAAIQKHMLWSNIVMDXXXXXXXXXXXXXKTEIICSWTFALVHYMTDSVLRSGCV 84
           V++A +AA+Q+H  WS + +D              TE +CS+ F      T+ +LRSG V
Sbjct: 196 VKHAEEAALQRHSTWSAMAVDLVLGNLIGLGLLFNTESVCSFVFDFAKEFTNGILRSGSV 255

Query: 85  WLMGVPAGFKLNTELAELLGMISLNAIQIYSTLWSIVGGFLRHIIWGLAFSGILLGLTVP 144
           WLMGVPAGFKLNTELA +LGM+SLN IQI+STLW  +  F+  +I  +A  GI  G TV 
Sbjct: 256 WLMGVPAGFKLNTELAGVLGMVSLNVIQIWSTLWVFMASFIFCLIRVIAILGITFGATVS 315

Query: 145 VSFFIDVIQLATLHVTLLQWLISLIYSRQIQTVASLWRLFR 185
            +F IDVI  ATLH+  L W I+L+YS QIQ +A+LWRLFR
Sbjct: 316 AAFVIDVITFATLHIMALHWAITLVYSHQIQALAALWRLFR 356



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.330    0.140    0.451 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,004,709
Number of extensions: 98825
Number of successful extensions: 308
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 308
Number of HSP's successfully gapped: 1
Length of query: 185
Length of database: 11,106,569
Length adjustment: 93
Effective length of query: 92
Effective length of database: 8,556,881
Effective search space: 787233052
Effective search space used: 787233052
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 108 (46.2 bits)