BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0274300 Os05g0274300|AK071399
         (382 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT4G13030.1  | chr4:7607400-7609656 FORWARD LENGTH=482            192   2e-49

>AT4G13030.1 | chr4:7607400-7609656 FORWARD LENGTH=482
          Length = 481

 Score =  192 bits (489), Expect = 2e-49,   Method: Compositional matrix adjust.
 Identities = 121/289 (41%), Positives = 170/289 (58%), Gaps = 19/289 (6%)

Query: 60  RLQDRSLREAKELIRRYKPGDLVEGVCGTKSGDYVLPDITTFLLVGPRDAGKSALVNRIT 119
           R   R LR+A+  I  Y PG   +     K  DY +P  T+ +LVGP+ AGKS+LVN+IT
Sbjct: 72  RTSKRKLRQARNEILSYTPGSWSD----VKLSDYNIPKTTSIMLVGPKGAGKSSLVNKIT 127

Query: 120 RVFXXXXXXXXXXRAQVSCNSKSTG-TSFLREYRVPRN-SNSICIYDTRSLSN-NHENNF 176
           RV           RAQ S  + S G T F++EY + R  S S C+YDTR LS  +  +N 
Sbjct: 128 RVIEDDAFLLD--RAQESFGTPSKGGTYFVQEYMISRGGSASFCLYDTRGLSRISSSDNT 185

Query: 177 KMLQRWMTKGLSHGDIITWDNDNYS-KIQNIKSMGRQYSFLRCKTRKVNFVIFVVNGASV 235
            M+++WMT+G+ HG+ + W +D+   K + I+  G  Y     + RKVN +IFVVN   +
Sbjct: 186 SMIEQWMTRGVLHGEPVIWASDSSDLKDRLIRDGGTGY-----ERRKVNSIIFVVNAVEI 240

Query: 236 LESIENNNKNYIDMLHKTFMYPFLSFGDDKPAVVVTHGDRLTAQQRMHVRNELVELLGIP 295
           L+S+E    +Y  M+   F  P L F DDKPAVV+THGD L+ ++R  VR  L ELLGIP
Sbjct: 241 LKSMECET-SYASMITTAFNSPILLFKDDKPAVVMTHGDMLSREERARVRVFLGELLGIP 299

Query: 296 L-QQIFDISGCDDYETDLSVLDMLRYCIQHAEQNFPI--KKNYLLEMHG 341
             +QIFDI    D  T +++ ++L Y +QHA++NF    KKN+ +   G
Sbjct: 300 PHKQIFDIPESRDTATAITICNLLCYSLQHADKNFVFLPKKNFTISKVG 348



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.323    0.139    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 7,896,644
Number of extensions: 324933
Number of successful extensions: 862
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 856
Number of HSP's successfully gapped: 1
Length of query: 382
Length of database: 11,106,569
Length adjustment: 100
Effective length of query: 282
Effective length of database: 8,364,969
Effective search space: 2358921258
Effective search space used: 2358921258
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 112 (47.8 bits)