BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os05g0200700 Os05g0200700|AK110596
(250 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT1G52140.1 | chr1:19407559-19408185 REVERSE LENGTH=209 60 1e-09
AT3G16330.1 | chr3:5535263-5535880 FORWARD LENGTH=206 54 8e-08
>AT1G52140.1 | chr1:19407559-19408185 REVERSE LENGTH=209
Length = 208
Score = 59.7 bits (143), Expect = 1e-09, Method: Compositional matrix adjust.
Identities = 56/224 (25%), Positives = 85/224 (37%), Gaps = 33/224 (14%)
Query: 16 MARRLWHVVLAVCHMLRRGLCRKRLMVDLHVXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 75
++++LW++V + +M+R+G+ + +L+ D +
Sbjct: 7 ISKKLWNIVRFLLYMIRKGVSKNKLIADFNATLKRGKNLMFHQRRRVHAGSTASAALNAT 66
Query: 76 XXXXXXXXXXXFYGRRPREVEFSCTTTPS-SYPHYGLFPFKSXXXXXXXXXXXXXXXXXX 134
+E EFSC+ TP+ S+P + F
Sbjct: 67 SATASSR----------QEYEFSCSNTPNYSFP-FSNMAFMRKKSHNNLFTCGQTPQTLD 115
Query: 135 LDAAAVARAFEMMSAEVEG---TPSSSAAAVQXXXXXXWATATPSPMVAWILGRSPAGVR 191
D AA E+++ E TP+ A+ SP G++P VR
Sbjct: 116 DDVAAARAVLELLNGVGEKGNVTPADLTVAL-------------SPYFPG-FGQTPL-VR 160
Query: 192 PLRVTDSPFPAVPENXXXXXXEQRVDDAEFEDFINKFYEQLRMQ 235
PLRVTDSPFP PEN VD A +DFI KFY+ L Q
Sbjct: 161 PLRVTDSPFPLTPEN--GDVANGHVDKAA-DDFIKKFYKNLNQQ 201
>AT3G16330.1 | chr3:5535263-5535880 FORWARD LENGTH=206
Length = 205
Score = 53.9 bits (128), Expect = 8e-08, Method: Compositional matrix adjust.
Identities = 51/231 (22%), Positives = 82/231 (35%), Gaps = 52/231 (22%)
Query: 16 MARRLWHVVLAVCHMLRRGLCRKRLMVDLHVXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 75
++++L ++V V +ML +G+ +++L+ D +
Sbjct: 7 ISKKLGNIVRFVLYMLHKGISKQKLLADFNATLKRGKNLM-------------------- 46
Query: 76 XXXXXXXXXXXFYGRR-----------PREVEFSCTTTPSSYPHYGLFPFKSXXXXXXXX 124
F+ RR E EFSC+ TP+ + + FK
Sbjct: 47 -----------FHNRRRVPGSAVASHPQNEYEFSCSDTPNYTFPFNMAAFKKKSHHNSLF 95
Query: 125 XXXXXXXXXXLDAAAVARAFEMMSAEVEGTPSSSAAAVQXXXXXXWATATPSPMVAWILG 184
D + E++++ + S+ A + P G
Sbjct: 96 SCGQAPPTLDDDTSVSRAVLELLNSGGDHDQGSNTPAFSIEALTALSPYLPG------FG 149
Query: 185 RSPAGVRPLRVTDSPFPAVPENXXXXXXEQRVDDAEFEDFINKFYEQLRMQ 235
RS + VRPLRVTDSPFP E VD A ++FI KFY+ L Q
Sbjct: 150 RSTSSVRPLRVTDSPFPLREEG---DVANGHVDKAA-DEFIKKFYKNLYQQ 196
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.324 0.134 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,827,170
Number of extensions: 108447
Number of successful extensions: 235
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 233
Number of HSP's successfully gapped: 2
Length of query: 250
Length of database: 11,106,569
Length adjustment: 96
Effective length of query: 154
Effective length of database: 8,474,633
Effective search space: 1305093482
Effective search space used: 1305093482
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 110 (47.0 bits)