BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0161400 Os05g0161400|AK105485
         (360 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G27660.1  | chr5:9789835-9792235 REVERSE LENGTH=429             55   8e-08

>AT5G27660.1 | chr5:9789835-9792235 REVERSE LENGTH=429
          Length = 428

 Score = 54.7 bits (130), Expect = 8e-08,   Method: Compositional matrix adjust.
 Identities = 72/290 (24%), Positives = 128/290 (44%), Gaps = 22/290 (7%)

Query: 22  KPKDIYTARD-VASTPDKLMIRKAARSVVAIETTYSDGKIIAVFSGIVVSWNETTRSATI 80
           KP + Y  RD +A+   ++       SV       S GK I   SG ++  +      TI
Sbjct: 108 KPSNGYLGRDTIANAAARIGPAVVNLSVPQGFHGISMGKSIG--SGTIIDAD-----GTI 160

Query: 81  VTCSEAVCD-DGALIDPKPKVLVHLPNNTILDGQLLFFNDHYRIMLLEVVSDTPLQPANF 139
           +TC+  V D        K +V V L +    +G ++  +    I L+++ S TPL  A  
Sbjct: 161 LTCAHVVVDFQNIRHSSKGRVDVTLQDGRTFEGVVVNADLQSDIALVKIKSKTPLPTAKL 220

Query: 140 GSTPKF--GQDVFALS--RDYESSMHARRGTVLWQEPPNVL---EYMYYCLSLSCQLAPC 192
           G + K   G  V A+      ++++ A   + + ++  ++    ++  Y L   C +   
Sbjct: 221 GFSSKLRPGDWVIAVGCPLSLQNTVTAGIVSCVDRKSSDLGLGGKHREY-LQTDCSINAG 279

Query: 193 GSGGSVIDQHGDVVGMAIGAPPNPDIL----PISIVQTCIEMWTKFSRIARPVLNMELRA 248
            SGG +++  G+V+G+ I      D L    PI  V   IE + K  R+ RP + +++  
Sbjct: 280 NSGGPLVNLDGEVIGVNIMKVLAADGLGFSVPIDSVSKIIEHFKKSGRVIRPWIGLKMVE 339

Query: 249 F-ELIEVSHQEEIELDHNINDGFIVAVVYDDSTAVRLGISQGDIILSYNG 297
              LI    +E   +  ++  G +V  V   S A R G   GD+++ ++G
Sbjct: 340 LNNLIVAQLKERDPMFPDVERGVLVPTVIPGSPADRAGFKPGDVVVRFDG 389



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.320    0.137    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 8,436,462
Number of extensions: 361419
Number of successful extensions: 692
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 691
Number of HSP's successfully gapped: 1
Length of query: 360
Length of database: 11,106,569
Length adjustment: 100
Effective length of query: 260
Effective length of database: 8,364,969
Effective search space: 2174891940
Effective search space used: 2174891940
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 112 (47.8 bits)