BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0143200 Os05g0143200|AK100211
         (163 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G27400.1  | chr5:9676362-9678285 FORWARD LENGTH=370            124   2e-29

>AT5G27400.1 | chr5:9676362-9678285 FORWARD LENGTH=370
          Length = 369

 Score =  124 bits (311), Expect = 2e-29,   Method: Compositional matrix adjust.
 Identities = 73/164 (44%), Positives = 98/164 (59%), Gaps = 9/164 (5%)

Query: 1   MKGNMEMNNLCVEQEGSQLPEENKN-KVQCKYLSWEETSESDLWDCRPDLVLGADIIYDP 59
           MK N+E N+L  + E  + P E ++ +V+C +L WE  SES+L   RPD+VLGAD+IYDP
Sbjct: 209 MKLNLERNHLNYDDEFLKQPGEAQSTRVKCTHLPWETASESELSQYRPDIVLGADVIYDP 268

Query: 60  VCVPHLIRVLSMLLXX--XXXXXXXXVANKEETSNEFSSAATEVPVAYIATVIRNAETFN 117
            C+PHL+RVL  LL              +++ T  E S A     VAYIA+VIRN +TFN
Sbjct: 269 SCLPHLLRVLVALLKNPPKRGNGSLETEDRDTTQEEHSPA-----VAYIASVIRNVDTFN 323

Query: 118 CFDKAAADAKLSVVNITNSEAP-LSLLPYMLSYDRSSVQLLKIT 160
                A    LS+ ++T    P   LLPYM SYDRS+V+L  I+
Sbjct: 324 VCLTLADQMDLSITDVTAELTPRFELLPYMHSYDRSTVRLFSIS 367



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.315    0.129    0.371 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,145,546
Number of extensions: 107262
Number of successful extensions: 250
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 248
Number of HSP's successfully gapped: 1
Length of query: 163
Length of database: 11,106,569
Length adjustment: 91
Effective length of query: 72
Effective length of database: 8,611,713
Effective search space: 620043336
Effective search space used: 620043336
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 107 (45.8 bits)