BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0122400 Os05g0122400|J033070L12
         (281 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G02880.1  | chr5:662643-668847 FORWARD LENGTH=1503             114   6e-26
AT4G38600.1  | chr4:18041503-18049292 REVERSE LENGTH=1889         108   3e-24

>AT5G02880.1 | chr5:662643-668847 FORWARD LENGTH=1503
          Length = 1502

 Score =  114 bits (285), Expect = 6e-26,   Method: Compositional matrix adjust.
 Identities = 55/115 (47%), Positives = 78/115 (67%), Gaps = 3/115 (2%)

Query: 164  NKDLDIYDIPSFDPKLGKTVMEFQALVKRKKFLEERASNPAA---DLSYKNVRLEDLCLD 220
             ++L  +DI   DP+L KT++E QALV+RKK   E   +  A   DLS+   ++EDLCL+
Sbjct: 1247 GQELSSFDIHFVDPELCKTLVELQALVRRKKLFAEAHGDSGAAKCDLSFHGTKIEDLCLE 1306

Query: 221  FTLPGNPEYELVPGGSEKMVTLDNLEEYVSSIVDATLKSGISNQIEAFKAGINKV 275
            F LPG  +Y+L P  +  MV LDNLEEY+  IV+AT+ +GI  Q+EAF++G N+V
Sbjct: 1307 FALPGYTDYDLAPYSANDMVNLDNLEEYIKGIVNATVCNGIQKQVEAFRSGFNQV 1361




 Score = 96.7 bits (239), Expect = 1e-20,   Method: Compositional matrix adjust.
 Identities = 51/158 (32%), Positives = 86/158 (54%)

Query: 4   PKECLSHGVPAAVLQFFDFFSMHKQKLVLKIVANVLGDFSXXXXXXXXXXXPVLCNLLQS 63
           P  CL+ G   AVL F DFFS   Q++ +  V N+    S           P+LC LLQ 
Sbjct: 229 PVACLNAGAIMAVLSFIDFFSTSIQRVAISTVVNICKQLSSESPSPFMDAVPILCTLLQY 288

Query: 64  TDKTILDSAVSCLVLVSDGACDSAQHMEKLYELNAVQATMRLMENDGWKSLSDETLSGIL 123
            D+ ++++   CL  ++D A +S   +++L     +  +  L+  +   +LS    +G++
Sbjct: 289 EDRQLVENVAICLTKIADQASESPAMLDQLCRHGLINESTHLLNLNSRTTLSQPVYNGVI 348

Query: 124 GLLKDLASLSARAVKSLFELNICDLLKQMITYYTSSHS 161
           G+L+ L+S SA A ++L+ELNI   LK++++ Y  SHS
Sbjct: 349 GMLRKLSSGSALAFRTLYELNIGYSLKEIMSTYDISHS 386



>AT4G38600.1 | chr4:18041503-18049292 REVERSE LENGTH=1889
          Length = 1888

 Score =  108 bits (270), Expect = 3e-24,   Method: Compositional matrix adjust.
 Identities = 55/115 (47%), Positives = 81/115 (70%), Gaps = 5/115 (4%)

Query: 164  NKDLDIYDIPSFDPKLGKTVMEFQALVKRKKFLEERA---SNPAADLSYKNVRLEDLCLD 220
             ++LD++DI  FD +LGKT+ E + +V RK +LE      S+  +DL  +  R+EDL L+
Sbjct: 1623 GQELDLHDIVLFDAELGKTLQELRVVVARKHYLEGVGGDNSSTISDLCLRGCRIEDLSLE 1682

Query: 221  FTLPGNPEYELVPGGSEKMVTLDNLEEYVSSIVDATLKSGISNQIEAFKAGINKV 275
            FTLPG PEY L  G  +++V + NLEEY+S +VDAT+K G++ QIEAF++G N+V
Sbjct: 1683 FTLPGYPEYILRSG--DEIVDITNLEEYISLVVDATVKRGVTRQIEAFRSGFNQV 1735




 Score = 58.5 bits (140), Expect = 4e-09,   Method: Compositional matrix adjust.
 Identities = 41/142 (28%), Positives = 61/142 (42%), Gaps = 4/142 (2%)

Query: 2   ECPKECLSHGVPAAVLQFFDFFSMHKQKLVLKIVANVLGDFSXXXXXXXXXXXPVLCNLL 61
           E P  CL  G   AVL + DFFS   Q++ L   AN+                P+L NLL
Sbjct: 311 EHPTACLRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDASDYVMEAVPLLTNLL 370

Query: 62  QSTDKTILDSAVSCLVLVSDGACDSAQHMEKLYELNAVQATMRLM----ENDGWKSLSDE 117
           Q  D  +L+ A  CL  +++      + +++L     V     L+       G  SLS  
Sbjct: 371 QYHDSKVLEYASICLTRIAEAFAPYPEKLDELCNHGLVTQAASLISTSNSGGGQASLSVS 430

Query: 118 TLSGILGLLKDLASLSARAVKS 139
           T +G++ LL   AS S    ++
Sbjct: 431 TYTGLIRLLSTCASGSPLGFRT 452



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.318    0.134    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 5,691,247
Number of extensions: 217636
Number of successful extensions: 598
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 594
Number of HSP's successfully gapped: 5
Length of query: 281
Length of database: 11,106,569
Length adjustment: 97
Effective length of query: 184
Effective length of database: 8,447,217
Effective search space: 1554287928
Effective search space used: 1554287928
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 111 (47.4 bits)