BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os05g0103500 Os05g0103500|AK060306
(180 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT4G12340.1 | chr4:7321492-7322004 REVERSE LENGTH=171 103 7e-23
>AT4G12340.1 | chr4:7321492-7322004 REVERSE LENGTH=171
Length = 170
Score = 103 bits (256), Expect = 7e-23, Method: Compositional matrix adjust.
Identities = 62/175 (35%), Positives = 93/175 (53%), Gaps = 9/175 (5%)
Query: 1 MGDYTIQISTKLIDQLARDDEXXXXXXXXXXXXXXXXQHQQEPQDNSRELPASEPKAPPG 60
MGD++IQIS+KLI+QLA ++ Q+ QD ++ P
Sbjct: 1 MGDFSIQISSKLINQLAEGNDQPKRRAKKTKPKVSPQSKQKTNQDEEKK-----PNPVAE 55
Query: 61 WPLQXXXXXXXXXXXXXXXXAFSELEAIRAVLEESEKVQEKLDKQHAGMRDELIKKSKDL 120
P+Q A +ELE+I++V++ESEKV EKL+ Q + E+ +++KDL
Sbjct: 56 LPMQPPFFFPIPPQGA----ASTELESIKSVVKESEKVLEKLELQEKNIVREVTERAKDL 111
Query: 121 RDKEFKLPYQNPTPCTEERSNCRQCYVSNAQDPLKCAEAVKRFEACVRLARQRGN 175
R+KEFK+P P PC+ + +CY N PLKC+ VK F+ C R +RQ+ N
Sbjct: 112 REKEFKIPEPKPMPCSSDHEAWMKCYKENIGSPLKCSGFVKSFQDCARRSRQQVN 166
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.313 0.128 0.372
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,194,133
Number of extensions: 107709
Number of successful extensions: 378
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 377
Number of HSP's successfully gapped: 1
Length of query: 180
Length of database: 11,106,569
Length adjustment: 92
Effective length of query: 88
Effective length of database: 8,584,297
Effective search space: 755418136
Effective search space used: 755418136
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 108 (46.2 bits)