BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0103100 Os05g0103100|AK103317
         (225 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G14030.1  | chr5:4526878-4528253 FORWARD LENGTH=196            158   2e-39

>AT5G14030.1 | chr5:4526878-4528253 FORWARD LENGTH=196
          Length = 195

 Score =  158 bits (400), Expect = 2e-39,   Method: Compositional matrix adjust.
 Identities = 69/136 (50%), Positives = 102/136 (75%)

Query: 20  AAAAGQDAPFVVAQKKVALSRPGPGVERLAVTLNLYNQGSATAYDVSLNDDSWPQEAFQL 79
           A+ A  + PF+V  KK  L+R   G ER++V+ ++YNQGS++AYDV+L D+SW ++ F++
Sbjct: 21  ASFATSEVPFMVVHKKATLNRLKSGAERVSVSYDIYNQGSSSAYDVTLTDNSWDKKTFEV 80

Query: 80  ISGTTSKIVEKLDPGATASHNFILETKVQGKFQGSPAIITYRVPTKAALQEAYSTPMFPL 139
           ++G TSK  E+LD G   SH+  LE KV+G F G+PA++T+R+PTK ALQEAYSTP+ PL
Sbjct: 81  VNGNTSKSWERLDAGGILSHSIELEAKVKGVFYGAPAVVTFRIPTKPALQEAYSTPLLPL 140

Query: 140 DILAERPPQQKFELVK 155
           DILA++PP +  ++ K
Sbjct: 141 DILADKPPTKPLDVAK 156



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.322    0.135    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,428,672
Number of extensions: 166468
Number of successful extensions: 265
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 265
Number of HSP's successfully gapped: 1
Length of query: 225
Length of database: 11,106,569
Length adjustment: 95
Effective length of query: 130
Effective length of database: 8,502,049
Effective search space: 1105266370
Effective search space used: 1105266370
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 110 (47.0 bits)