BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0682000 Os04g0682000|AK069012
         (478 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G59950.1  | chr3:22144468-22146887 REVERSE LENGTH=478          431   e-121
AT2G44140.1  | chr2:18255106-18257165 REVERSE LENGTH=468          425   e-119

>AT3G59950.1 | chr3:22144468-22146887 REVERSE LENGTH=478
          Length = 477

 Score =  431 bits (1107), Expect = e-121,   Method: Compositional matrix adjust.
 Identities = 212/401 (52%), Positives = 280/401 (69%), Gaps = 17/401 (4%)

Query: 85  VCSGSMWRF----LGTSKV---LTSSDVWFLGKCYKLXXXXXXXXXXXXXGHATFLEDFS 137
           + SG++ RF    LG S+     ++S++W LG CYK+               A F +DFS
Sbjct: 87  MASGAIRRFQDRVLGPSRTGISSSTSEIWLLGVCYKISEGESSEEADAGRVLAAFRQDFS 146

Query: 138 SRIWITYRRGFDAISDSKYTSDVNWGCMVRSSQMLVAQALIFHHLGRSWRRPLEKPYNPE 197
           S I +TYRRGF+ I D+ YTSDVNWGCM+RS QML AQAL+F  LGRSWR+   +P + +
Sbjct: 147 SLILMTYRRGFEPIGDTTYTSDVNWGCMLRSGQMLFAQALLFQRLGRSWRKKDSEPADEK 206

Query: 198 YIGILHMFGDSEACAFSIHNLLQAGNSYGLAAGSWVGPYAMCRAWQTLVRTNREQHEVVD 257
           Y+ IL +FGD+EA AFSIHNL+ AG SYGLAAGSWVGPYA+CR+W++L R N+E  E  D
Sbjct: 207 YLEILELFGDTEASAFSIHNLILAGESYGLAAGSWVGPYAVCRSWESLARKNKE--ETDD 264

Query: 258 GNESFPMALYVVSGDEDGERGGAPVVCIDVAAQLCCDFNKGQSTWSPIXXXXXXXXXXDK 317
            ++SF MA+++VSG EDGERGGAP++CI+   + C +F++G++ W PI          D+
Sbjct: 265 KHKSFSMAVHIVSGSEDGERGGAPILCIEDVTKTCLEFSEGETEWPPILLLVPLVLGLDR 324

Query: 318 INPRYIPLLKETFTFPQSLGILGGKPGTSTYIAGVQDDRALYLDPHEVQMAVDIAADNIE 377
           +NPRYIP L  TFTFPQSLGILGGKPG STYI GVQ+D+  YLDPH+VQ  V +  +N +
Sbjct: 325 VNPRYIPSLIATFTFPQSLGILGGKPGASTYIVGVQEDKGFYLDPHDVQQVVTVKKENQD 384

Query: 378 ADTSSYHCSTVRDLALDLIDPSLAIGFYCRDKDDFDDFCSRATELVDKANGAPLFTVVQS 437
            DTSSYHC+T+R + L+ +DPSLA+GFYC+ KDDFDDFC RAT+L   +NGAPLFTV QS
Sbjct: 385 VDTSSYHCNTLRYVPLESLDPSLALGFYCQHKDDFDDFCIRATKLAGDSNGAPLFTVTQS 444

Query: 438 VQPSKQMYNQDDVLGISGDGNINVEDLDASGETGEEEWQIL 478
                  + ++D  GI+   +      + SGE  E++WQ+L
Sbjct: 445 -------HRRNDC-GIAETSSSTETSTEISGEEHEDDWQLL 477



>AT2G44140.1 | chr2:18255106-18257165 REVERSE LENGTH=468
          Length = 467

 Score =  425 bits (1093), Expect = e-119,   Method: Compositional matrix adjust.
 Identities = 218/442 (49%), Positives = 287/442 (64%), Gaps = 18/442 (4%)

Query: 4   LPDRGVSSSSSDPLCEGNIAPCSSSSEQKEDCSLKQSKTSILSCVFNSPFNIFEAHQDXX 63
           +P +  SSS SD   +  +   S  S+ K       SK ++ S VF S  ++ + +++  
Sbjct: 9   VPQQCSSSSKSDTHDKSPLVSDSGPSDNK-------SKFTLWSNVFTSSSSVSQPYRESS 61

Query: 64  XXXXXXXXXXXXXXXRVLRRI-VCSGSMWRF----LGTSKV---LTSSDVWFLGKCYKLX 115
                            ++R+ + SG++ RF    LG ++     T+SDVW LG CYK+ 
Sbjct: 62  TSGHKQVCTTRNGWTAFVKRVSMASGAIRRFQERVLGPNRTGLPSTTSDVWLLGVCYKIS 121

Query: 116 XXXXXXXXXXXXGHATFLEDFSSRIWITYRRGFDAISDSKYTSDVNWGCMVRSSQMLVAQ 175
                         A    DFSS+I +TYR+GF+   D+ YTSDVNWGCM+RSSQML AQ
Sbjct: 122 ADENSGETDTGTVLAALQLDFSSKILMTYRKGFEPFRDTTYTSDVNWGCMIRSSQMLFAQ 181

Query: 176 ALIFHHLGRSWRRPLEKPYNPEYIGILHMFGDSEACAFSIHNLLQAGNSYGLAAGSWVGP 235
           AL+FH LGR+W +  E P   EY+  L  FGDSE  AFSIHNL+ AG SYGLAAGSWVGP
Sbjct: 182 ALLFHRLGRAWTKKSELPEQ-EYLETLEPFGDSEPSAFSIHNLIIAGASYGLAAGSWVGP 240

Query: 236 YAMCRAWQTLVRTNREQHEVVDGNESFPMALYVVSGDEDGERGGAPVVCIDVAAQLCCDF 295
           YA+CRAW++L    R+Q +    N++ PMA+++VSG EDGERGGAP++CI+ A + C +F
Sbjct: 241 YAICRAWESLACKKRKQTD--SKNQTLPMAVHIVSGSEDGERGGAPILCIEDATKSCLEF 298

Query: 296 NKGQSTWSPIXXXXXXXXXXDKINPRYIPLLKETFTFPQSLGILGGKPGTSTYIAGVQDD 355
           +KGQS W+PI          D +NPRYIP L  TFTFPQS+GILGGKPG STYI GVQ+D
Sbjct: 299 SKGQSEWTPIILLVPLVLGLDSVNPRYIPSLVATFTFPQSVGILGGKPGASTYIVGVQED 358

Query: 356 RALYLDPHEVQMAVDIAADNIEADTSSYHCSTVRDLALDLIDPSLAIGFYCRDKDDFDDF 415
           +  YLDPHEVQ  V +  +  + DTSSYHC+ +R + L+ +DPSLA+GFYCRDKDDFDDF
Sbjct: 359 KGFYLDPHEVQQVVTVNKETPDVDTSSYHCNVLRYVPLESLDPSLALGFYCRDKDDFDDF 418

Query: 416 CSRATELVDKANGAPLFTVVQS 437
           C RA +L +++NGAPLFTV Q+
Sbjct: 419 CLRALKLAEESNGAPLFTVTQT 440



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.319    0.135    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 10,418,608
Number of extensions: 416087
Number of successful extensions: 892
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 886
Number of HSP's successfully gapped: 2
Length of query: 478
Length of database: 11,106,569
Length adjustment: 102
Effective length of query: 376
Effective length of database: 8,310,137
Effective search space: 3124611512
Effective search space used: 3124611512
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 114 (48.5 bits)