BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0674400 Os04g0674400|AK102124
         (265 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G18400.2  | chr5:6092955-6094876 REVERSE LENGTH=273            190   5e-49
AT5G18362.1  | chr5:6083088-6084362 REVERSE LENGTH=183             73   2e-13

>AT5G18400.2 | chr5:6092955-6094876 REVERSE LENGTH=273
          Length = 272

 Score =  190 bits (483), Expect = 5e-49,   Method: Compositional matrix adjust.
 Identities = 119/268 (44%), Positives = 149/268 (55%), Gaps = 15/268 (5%)

Query: 8   ALAVTDELALPLRAVGDLAAAAGVSREEV-----VVITQCASLGGKLPFDDASVGSVLAV 62
            LAVTD++ LP+ +V  LA    + +E +     ++ITQ AS   + P D +SV +VLA+
Sbjct: 10  VLAVTDDVVLPVSSV--LAIMKELGKEVIESFDPLIITQ-ASTINQFPLDASSVEAVLAI 66

Query: 63  IKKVENLGDLFITEISRVLKAGGMVLIQSSPSDQ--DPNNSIQRKLLLGGFXX---XXXX 117
            K  +   D    E SR+LK GG V +      +  +   +IQR++ L GF         
Sbjct: 67  SKTSDFPSDKICGEFSRILKPGGTVSVCKVLEGETGEIQQTIQRRVTLAGFLEPQCLDLK 126

Query: 118 XXXXXXXEHSVTIKAKKVSWSLGSSFPLKKATKGLPKIQXXXXXXXXXXXXXXXXXXXKK 177
                    S  IKAKK SW +GSSF LKK    L KI                     K
Sbjct: 127 SIKLSTFSLSFGIKAKKPSWKIGSSFALKKPVTNLFKIDLDDDVDLIDEDSLLTEEDLMK 186

Query: 178 PELPVVGDCEVGATRKACKNCTCGRAEAEEKVEKLNLTSEQINNPQSACGNCGLGDAFRC 237
           P+LPV   CE   T+KACKNC CGRAE EEK  KL LT +QI NPQS+CG+CGLGDAFRC
Sbjct: 187 PQLPVASGCE--TTKKACKNCVCGRAEIEEKAVKLGLTEDQIENPQSSCGSCGLGDAFRC 244

Query: 238 GTCPYRGLPAFKPGEKIALPGNFLAADM 265
           GTCPY+GLP FK GEK+ L  NFL AD+
Sbjct: 245 GTCPYKGLPPFKLGEKVTLSQNFLEADI 272



>AT5G18362.1 | chr5:6083088-6084362 REVERSE LENGTH=183
          Length = 182

 Score = 73.2 bits (178), Expect = 2e-13,   Method: Compositional matrix adjust.
 Identities = 44/96 (45%), Positives = 51/96 (53%), Gaps = 2/96 (2%)

Query: 133 KKVSWSLGSSFPLKKATKGLPKIQXXXXXXXXXXXXXXXXXXXKKPELPVVGDCEVGATR 192
           KK SW +GSSF LKK  K L K                     KKP++P    CE    +
Sbjct: 13  KKNSWKIGSSFALKKPAKFLLKDNLDDDLDLIDEDSLLTEDDLKKPQVPAASGCET--PK 70

Query: 193 KACKNCTCGRAEAEEKVEKLNLTSEQINNPQSACGN 228
           KACKNC CGR E E K  KL LT +QI NPQS+CG+
Sbjct: 71  KACKNCVCGRDEIERKAVKLGLTEDQIENPQSSCGS 106



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.316    0.133    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,841,829
Number of extensions: 167552
Number of successful extensions: 488
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 483
Number of HSP's successfully gapped: 2
Length of query: 265
Length of database: 11,106,569
Length adjustment: 97
Effective length of query: 168
Effective length of database: 8,447,217
Effective search space: 1419132456
Effective search space used: 1419132456
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 111 (47.4 bits)