BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0659900 Os04g0659900|AK060780
         (100 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT4G27650.1  | chr4:13803459-13807556 REVERSE LENGTH=379          155   4e-39
AT3G58390.1  | chr3:21600030-21601217 REVERSE LENGTH=396          152   4e-38

>AT4G27650.1 | chr4:13803459-13807556 REVERSE LENGTH=379
          Length = 378

 Score =  155 bits (392), Expect = 4e-39,   Method: Compositional matrix adjust.
 Identities = 75/101 (74%), Positives = 84/101 (83%), Gaps = 4/101 (3%)

Query: 4   LHPFH----IDSARACYGPKHVEIANERLAIQTLLITDNLFRNSDIATRQNYVRLVESVK 59
           L+ FH     +  RACYGPKHVE+ANER+AIQTLLITD LFRNSD+ TR+ YV LVESVK
Sbjct: 278 LNDFHNMLSTEPDRACYGPKHVEVANERMAIQTLLITDELFRNSDVKTRKKYVNLVESVK 337

Query: 60  KFGGTVHIFSSMHVSGEQLAQLTGIAAILRFPLPDLEDIEM 100
             GG   IFS+MHVSGEQLAQLTGIAA+LRFPLP+LEDIEM
Sbjct: 338 DSGGDAFIFSAMHVSGEQLAQLTGIAALLRFPLPELEDIEM 378



>AT3G58390.1 | chr3:21600030-21601217 REVERSE LENGTH=396
          Length = 395

 Score =  152 bits (384), Expect = 4e-38,   Method: Compositional matrix adjust.
 Identities = 74/100 (74%), Positives = 79/100 (79%)

Query: 1   NILLHPFHIDSARACYGPKHVEIANERLAIQTLLITDNLFRNSDIATRQNYVRLVESVKK 60
           N     F  D  RACYGPKHVE+A+ER+AIQTLLI D LFRNSD+ TR+ YV  VESVK 
Sbjct: 296 NDFFTMFSNDPNRACYGPKHVEVAHERMAIQTLLIIDGLFRNSDVKTRKKYVDFVESVKD 355

Query: 61  FGGTVHIFSSMHVSGEQLAQLTGIAAILRFPLPDLEDIEM 100
            GG V IFSSMH SGEQLAQ TGIAAILRFPLPDLEDIEM
Sbjct: 356 SGGEVFIFSSMHASGEQLAQHTGIAAILRFPLPDLEDIEM 395



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.324    0.139    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 1,949,776
Number of extensions: 60279
Number of successful extensions: 147
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 147
Number of HSP's successfully gapped: 2
Length of query: 100
Length of database: 11,106,569
Length adjustment: 70
Effective length of query: 30
Effective length of database: 9,187,449
Effective search space: 275623470
Effective search space used: 275623470
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 104 (44.7 bits)