BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os04g0653400 Os04g0653400|J080303E23
(141 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT4G24520.1 | chr4:12663065-12667066 REVERSE LENGTH=693 182 4e-47
AT4G30210.1 | chr4:14796900-14800578 FORWARD LENGTH=713 167 2e-42
AT3G02280.1 | chr3:453646-457659 FORWARD LENGTH=624 54 3e-08
>AT4G24520.1 | chr4:12663065-12667066 REVERSE LENGTH=693
Length = 692
Score = 182 bits (463), Expect = 4e-47, Method: Compositional matrix adjust.
Identities = 93/141 (65%), Positives = 104/141 (73%), Gaps = 2/141 (1%)
Query: 1 ETVEQAEKLLDLSPDTFFSVHADAEDGSPRKGGGSLAPPFPSPCTLRTALLRYADLLNSP 60
E VE+A KLL S D FS+HAD EDGSP + ++ PPFP PCTL T L RYADLLN P
Sbjct: 341 EIVEEAGKLLGHSLDLVFSIHADKEDGSPLES--AVPPPFPGPCTLGTGLARYADLLNPP 398
Query: 61 KKXXXXXXXXXXXXXXXXERLRFLASPAGKDEYSQWVVASQRSLLEVMAAFPSAKPPLGV 120
+K E+L+ L SP GKDEYSQW+VASQRSLLEVMAAFPSAKPPLGV
Sbjct: 399 RKSALVALAAYATEPSEAEKLKHLTSPDGKDEYSQWIVASQRSLLEVMAAFPSAKPPLGV 458
Query: 121 FFAAVAPRLQPRYYSISSSPK 141
FFAA+APRLQPRYYSISSSP+
Sbjct: 459 FFAAIAPRLQPRYYSISSSPR 479
>AT4G30210.1 | chr4:14796900-14800578 FORWARD LENGTH=713
Length = 712
Score = 167 bits (422), Expect = 2e-42, Method: Compositional matrix adjust.
Identities = 94/142 (66%), Positives = 106/142 (74%), Gaps = 4/142 (2%)
Query: 1 ETVEQAEKLLDLSPDTFFSVHADAEDGSPRKGGGSLAPPFPSPCTLRTALLRYADLLNSP 60
ETV++A +LLD+SPDT+FS+HA+ EDG+P S PP PC LRTAL RYA LL+SP
Sbjct: 361 ETVDEALRLLDMSPDTYFSLHAEKEDGTPIS---SSLPPPFPPCNLRTALTRYACLLSSP 417
Query: 61 KKXXXXXXXXXXXXXXXXERLRFLASPAGK-DEYSQWVVASQRSLLEVMAAFPSAKPPLG 119
KK ERL+ LASPAGK DEYS+WVV SQRSLLEVMA FPSAKPPLG
Sbjct: 418 KKSALVALAAHASDPTEAERLKHLASPAGKVDEYSKWVVESQRSLLEVMAEFPSAKPPLG 477
Query: 120 VFFAAVAPRLQPRYYSISSSPK 141
VFFA VAPRLQPR+YSISSSPK
Sbjct: 478 VFFAGVAPRLQPRFYSISSSPK 499
>AT3G02280.1 | chr3:453646-457659 FORWARD LENGTH=624
Length = 623
Score = 53.9 bits (128), Expect = 3e-08, Method: Compositional matrix adjust.
Identities = 33/99 (33%), Positives = 52/99 (52%), Gaps = 2/99 (2%)
Query: 43 PCTLRTALLRYADLLN-SPKKXXXXXXXXXXXXXXXXERLRFLASPAGKDEYSQWVVASQ 101
P L+T + D+ + SP++ ERL++ ASP G+D+ + +
Sbjct: 310 PIKLKTFVELTMDVTSASPRRYFFEIMSFYATAEHEKERLQYFASPEGRDDLYNYNQKER 369
Query: 102 RSLLEVMAAFPSAKPPLGVFFAAVAPRLQPRYYSISSSP 140
RS+LEV+ FPS + P + + P L+PR +SISSSP
Sbjct: 370 RSILEVLEDFPSVQIPFD-WLVQLVPPLKPRAFSISSSP 407
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.316 0.132 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 2,975,832
Number of extensions: 104185
Number of successful extensions: 213
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 207
Number of HSP's successfully gapped: 3
Length of query: 141
Length of database: 11,106,569
Length adjustment: 89
Effective length of query: 52
Effective length of database: 8,666,545
Effective search space: 450660340
Effective search space used: 450660340
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 106 (45.4 bits)