BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0628300 Os04g0628300|Os04g0628300
         (222 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G61760.1  | chr1:22807440-22808114 REVERSE LENGTH=225           77   6e-15
AT4G05220.1  | chr4:2685104-2685784 REVERSE LENGTH=227             73   1e-13

>AT1G61760.1 | chr1:22807440-22808114 REVERSE LENGTH=225
          Length = 224

 Score = 77.4 bits (189), Expect = 6e-15,   Method: Compositional matrix adjust.
 Identities = 49/165 (29%), Positives = 79/165 (47%), Gaps = 10/165 (6%)

Query: 59  RPHRPRFHVAAFTAAGVQ--SGGGPVALSGQLTIHNPNHDLAFFFGRVYMSVQYRGDGEV 116
           +PHRPR H+  F+ +G+    G     +S ++T HNPN ++  ++  +  SV Y+   E 
Sbjct: 67  QPHRPRVHIRGFSISGLSRPDGFETSHISFKITAHNPNQNVGIYYDSMEGSVYYK---EK 123

Query: 117 VVDGKDLTGGPLYEPPRGTSAVGFEGVAVPXXXXXXXXXXXXXXXXXXXXXXXXXFTVKV 176
            +    LT  P Y+ P+ TS++    ++ P                         F +KV
Sbjct: 124 RIGSTKLT-NPFYQDPKNTSSID-GALSRP---AMAVNKDRWMEMERDRNQGKIMFRLKV 178

Query: 177 RSRIRVRVAFWGSHWHPVHATCDVVVGPDGQLLPEFQQKRCGIDF 221
           RS IR +V  W S  H ++A+C + +G DG LL   + KRC + F
Sbjct: 179 RSMIRFKVYTWHSKSHKMYASCYIEIGWDGMLLSATKDKRCPVYF 223



>AT4G05220.1 | chr4:2685104-2685784 REVERSE LENGTH=227
          Length = 226

 Score = 73.2 bits (178), Expect = 1e-13,   Method: Compositional matrix adjust.
 Identities = 49/167 (29%), Positives = 75/167 (44%), Gaps = 14/167 (8%)

Query: 59  RPHRPRFHVAAFTAAGVQ--SGGGPVALSGQLTIHNPNHDLAFFFGRVYMSVQYRGDGEV 116
           RPHRPRFH+  F   G+   +G     ++  +TI NPN  +  +F  +  S+ Y+ D  V
Sbjct: 69  RPHRPRFHIQDFVVQGLDQPTGVENARIAFNVTILNPNQHMGVYFDSMEGSIYYK-DQRV 127

Query: 117 VVDGKDLTGGPLYEPPRGTSAVG--FEGVAVPXXXXXXXXXXXXXXXXXXXXXXXXXFTV 174
              G      P ++ P  T+ V     G ++                          F +
Sbjct: 128 ---GLIPLLNPFFQQPTNTTIVTGTLTGASL------TVNSNRWTEFSNDRAQGTVGFRL 178

Query: 175 KVRSRIRVRVAFWGSHWHPVHATCDVVVGPDGQLLPEFQQKRCGIDF 221
            + S IR ++  W S  H +HA C++VVG DG +LP+F  KRC + F
Sbjct: 179 DIVSTIRFKLHRWISKHHRMHANCNIVVGRDGLILPKFNHKRCPVYF 225



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.324    0.142    0.463 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,123,286
Number of extensions: 136831
Number of successful extensions: 292
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 291
Number of HSP's successfully gapped: 2
Length of query: 222
Length of database: 11,106,569
Length adjustment: 95
Effective length of query: 127
Effective length of database: 8,502,049
Effective search space: 1079760223
Effective search space used: 1079760223
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 110 (47.0 bits)