BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0625100 Os04g0625100|AK064614
         (627 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G20490.1  | chr3:7154178-7156382 REVERSE LENGTH=459            103   2e-22

>AT3G20490.1 | chr3:7154178-7156382 REVERSE LENGTH=459
          Length = 458

 Score =  103 bits (258), Expect = 2e-22,   Method: Compositional matrix adjust.
 Identities = 87/333 (26%), Positives = 138/333 (41%), Gaps = 65/333 (19%)

Query: 315 LMNHGVLMSQSTSKSKKFAQTPNYSASKSMEESSTKKLLPKTALSPMRKIHLLXXXXXX- 373
           L   GVL +Q +    K  Q+ +  AS +   SS   L   ++ SP+R+  LL       
Sbjct: 125 LHGSGVLSNQPSISRGKRKQS-DVQASAASGISSVASLFQMSSRSPLRRFQLLDSDSEDD 183

Query: 374 --XXNKEMPGLQQN----CKSKVSTVQHKGKAE------MNDSWA----------TPALD 411
               ++++ G  +      K++ S      + E      + D W           TPA D
Sbjct: 184 HPSTSRDLSGATKKHDSFSKNQPSIASKPKRKEPGSIPCIKDLWKDFSPASSKIQTPAFD 243

Query: 412 EFCNEYFKSVEDSRPSQQKEGNSFCGPKVIRSNYSVSETGGHFPHQSTPSGAVLEDNQTD 471
           + C +YF S++ +  +Q++             N +  +    F   S PS          
Sbjct: 244 DVCQDYFISIKTTSTAQKQSSAVASSSNSGNHNLTGFQQTELFHDFSHPS---------- 293

Query: 472 SHPPAMHYFFHHDQLVRDLVRQRLKHFVPVGVDSRGNEQDGTQNLQYRSQTG-------- 523
             PP+  +F H D  +R+L RQRL +F P+G+ +    Q     + Y +Q G        
Sbjct: 294 --PPSHRFFLHSDPRIRNLARQRLPNFFPLGIVNDRESQREVFLVDYMNQFGSKGSSKAG 351

Query: 524 --------------------RCAAENDRWVTPNKRMPVATQVGRRRVNPAGMSGSGHWLT 563
                                 A  ++ W+ P  R       G+RRV+ A    +GHW T
Sbjct: 352 DSSSKSCRRGKTKSKVSKSQESAHNSEGWLNPKTRAAAPKDAGKRRVS-ADSGSAGHWFT 410

Query: 564 GDDGKKVYISKDGQELTGRVAYRQYQRESGKGF 596
             +G+KVYISK GQE +G+ AYR Y++E+G GF
Sbjct: 411 SPEGRKVYISKSGQEFSGQSAYRCYKKENGVGF 443



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.312    0.129    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 13,034,745
Number of extensions: 568914
Number of successful extensions: 1145
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 1143
Number of HSP's successfully gapped: 2
Length of query: 627
Length of database: 11,106,569
Length adjustment: 105
Effective length of query: 522
Effective length of database: 8,227,889
Effective search space: 4294958058
Effective search space used: 4294958058
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 115 (48.9 bits)