BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os04g0539800 Os04g0539800|J033065A17
(115 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT4G34660.1 | chr4:16545595-16548294 REVERSE LENGTH=369 145 3e-36
AT4G18060.1 | chr4:10027668-10029662 REVERSE LENGTH=352 89 4e-19
AT4G39020.1 | chr4:18182315-18184340 FORWARD LENGTH=170 89 6e-19
AT1G31440.1 | chr1:11256150-11258479 REVERSE LENGTH=440 74 1e-14
>AT4G34660.1 | chr4:16545595-16548294 REVERSE LENGTH=369
Length = 368
Score = 145 bits (367), Expect = 3e-36, Method: Compositional matrix adjust.
Identities = 68/115 (59%), Positives = 85/115 (73%), Gaps = 1/115 (0%)
Query: 1 MVSERQKIEAPPTPSAENYMAQPPPSYDEVNGMFAXXXXXXXXXXXXFFLGEALDSFKAE 60
MVSERQ+IEAP TPS+ + M PPPSY+E NG+FA +FLGE L +
Sbjct: 255 MVSERQRIEAPSTPSSADSMP-PPPSYEEANGVFASQMHDTSTDSMGYFLGEVLFPYHGV 313
Query: 61 SESELNLSAGDIVIVRKISTNGWAEGECKGKAGWFPHGYIERRERVLASKVPHIF 115
++ EL+LS G+ V+VRK++ +GWAEGECKGKAGWFP+GYIERRERVLASKV +F
Sbjct: 314 TDVELSLSTGEYVVVRKVTGSGWAEGECKGKAGWFPYGYIERRERVLASKVSEVF 368
>AT4G18060.1 | chr4:10027668-10029662 REVERSE LENGTH=352
Length = 351
Score = 89.4 bits (220), Expect = 4e-19, Method: Compositional matrix adjust.
Identities = 36/59 (61%), Positives = 45/59 (76%)
Query: 48 FFLGEALDSFKAESESELNLSAGDIVIVRKISTNGWAEGECKGKAGWFPHGYIERRERV 106
+FL E + F A SE EL+L GD ++VRK+S GWAEGECKGKAGWFP YIE+R+R+
Sbjct: 283 YFLAEVIHPFSAASEKELDLDKGDYIVVRKVSQTGWAEGECKGKAGWFPMAYIEKRQRL 341
>AT4G39020.1 | chr4:18182315-18184340 FORWARD LENGTH=170
Length = 169
Score = 88.6 bits (218), Expect = 6e-19, Method: Compositional matrix adjust.
Identities = 37/68 (54%), Positives = 54/68 (79%)
Query: 48 FFLGEALDSFKAESESELNLSAGDIVIVRKISTNGWAEGECKGKAGWFPHGYIERRERVL 107
+FLGE + ++A+S+ EL+LS GD V++R++ ++ WAEGECKG AGWF + YIERR+RV
Sbjct: 102 YFLGEVMFPYQADSDFELSLSVGDYVVIREVVSSVWAEGECKGNAGWFTYIYIERRDRVF 161
Query: 108 ASKVPHIF 115
A+KV +F
Sbjct: 162 ATKVIEVF 169
>AT1G31440.1 | chr1:11256150-11258479 REVERSE LENGTH=440
Length = 439
Score = 74.3 bits (181), Expect = 1e-14, Method: Compositional matrix adjust.
Identities = 31/64 (48%), Positives = 47/64 (73%)
Query: 48 FFLGEALDSFKAESESELNLSAGDIVIVRKISTNGWAEGECKGKAGWFPHGYIERRERVL 107
+FL + + F A++ EL+L+ D VIVR+++ GW+EGE KGKAGWFP Y+E++E+
Sbjct: 368 YFLAKVVHPFDAQAPGELSLAVDDYVIVRQVAGTGWSEGEYKGKAGWFPSAYVEKQEKAP 427
Query: 108 ASKV 111
ASK+
Sbjct: 428 ASKI 431
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.315 0.133 0.402
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 2,336,062
Number of extensions: 74876
Number of successful extensions: 395
Number of sequences better than 1.0e-05: 4
Number of HSP's gapped: 394
Number of HSP's successfully gapped: 4
Length of query: 115
Length of database: 11,106,569
Length adjustment: 84
Effective length of query: 31
Effective length of database: 8,803,625
Effective search space: 272912375
Effective search space used: 272912375
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 104 (44.7 bits)