BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0534300 Os04g0534300|AK065842
         (288 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G73440.1  | chr1:27611418-27612182 FORWARD LENGTH=255          108   3e-24
AT4G00140.1  | chr4:51166-53449 REVERSE LENGTH=258                 60   2e-09

>AT1G73440.1 | chr1:27611418-27612182 FORWARD LENGTH=255
          Length = 254

 Score =  108 bits (270), Expect = 3e-24,   Method: Compositional matrix adjust.
 Identities = 48/89 (53%), Positives = 68/89 (76%), Gaps = 1/89 (1%)

Query: 195 AKTMRIKK-QGKSRIQLTEDDVVAFFFSFDEVGKGYITPWDLERMATVHDFIWTDSEISK 253
           A TM+ K+ +  S+ Q+T+D++V +F  FDE GKG+IT  D+ +MATVHDF WT+ E+  
Sbjct: 164 AATMKKKRPELMSKTQMTQDELVMYFCQFDEGGKGFITLRDVAKMATVHDFTWTEEELQD 223

Query: 254 MIHCFDSDRDGKINLEDFRTIVSRCNMLQ 282
           MI CFD D+DGK++L++FR IVSRC ML+
Sbjct: 224 MIRCFDMDKDGKLSLDEFRKIVSRCRMLK 252



>AT4G00140.1 | chr4:51166-53449 REVERSE LENGTH=258
          Length = 257

 Score = 59.7 bits (143), Expect = 2e-09,   Method: Compositional matrix adjust.
 Identities = 25/43 (58%), Positives = 32/43 (74%)

Query: 224 EVGKGYITPWDLERMATVHDFIWTDSEISKMIHCFDSDRDGKI 266
           E GKG+IT  D+ +MATVHDF WT+ E+  MI  FD D+DGK+
Sbjct: 91  EGGKGFITRRDVAKMATVHDFTWTEEELQDMIRSFDMDKDGKV 133



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.314    0.131    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,736,171
Number of extensions: 165277
Number of successful extensions: 502
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 530
Number of HSP's successfully gapped: 2
Length of query: 288
Length of database: 11,106,569
Length adjustment: 98
Effective length of query: 190
Effective length of database: 8,419,801
Effective search space: 1599762190
Effective search space used: 1599762190
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 111 (47.4 bits)