BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0528400 Os04g0528400|AK101159
         (326 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G52530.1  | chr1:19569045-19570004 FORWARD LENGTH=320          362   e-100

>AT1G52530.1 | chr1:19569045-19570004 FORWARD LENGTH=320
          Length = 319

 Score =  362 bits (930), Expect = e-100,   Method: Compositional matrix adjust.
 Identities = 178/326 (54%), Positives = 231/326 (70%), Gaps = 8/326 (2%)

Query: 1   MKFKAFFTDDGIALLDKRFLPAMDKVGRLCHVYLTPTHAMLLHNLLXXXXXXXXXXXXQC 60
           MKFKAF T++G+ LL+KRFLPA DK+G+ CH+ LT  H   LHNLL            QC
Sbjct: 1   MKFKAFLTENGVNLLEKRFLPAFDKMGKNCHLLLTKEHLFFLHNLLNGDGV-------QC 53

Query: 61  VAQFAKDLLFREYSVSSRNGNRVAFAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXIQVKL 120
           +AQF KD+LF +Y +SS+N +R+AF++D                           +Q+KL
Sbjct: 54  IAQFRKDVLFDDYRISSQNEDRIAFSLDVALLYRAVKSSVSICTEFSGGLASNR-LQIKL 112

Query: 121 VNKLPAGSRSASPFLTFETKGARSAVVQDVPISRPLSRSDVERLQAALDSAQELPQTLVQ 180
           V KLP       PFLTFETKG +SAV+QDVPI++PLSRS V  LQ ALDSAQ+LP TLVQ
Sbjct: 113 VKKLPPNCTQPMPFLTFETKGYKSAVIQDVPITKPLSRSQVIELQTALDSAQDLPPTLVQ 172

Query: 181 VPDLPQLQSLVDRLKNVGDLLTVAVTQYGDLHLHVATSLVTVGSEFRKLRILGVRATAPV 240
           V D  QLQ+ VD +++VGD+L V ++++GDL + V+T+L+ +G EF++L ++G ++ APV
Sbjct: 173 VQDSNQLQNFVDHMRHVGDVLNVTISKHGDLQVQVSTTLIRLGIEFQRLSVIGEKSQAPV 232

Query: 241 GDQNLSASTRTNMAIERGEALSVQVNMKHLAKSLQCHLAKPDCTFYGISPGGACLTVIFQ 300
            D+NLSA  R+  AI RG+A SVQV++KH +KSLQCHL KPD TFYGI+P GACLTVIFQ
Sbjct: 233 EDRNLSAQARSERAIARGDAQSVQVSVKHFSKSLQCHLTKPDSTFYGIAPQGACLTVIFQ 292

Query: 301 YFIPGTRLTDKSISFYCRLPVLDPGS 326
           + +PGTR TDKSIS +CRLPVLDPGS
Sbjct: 293 FMVPGTRQTDKSISLHCRLPVLDPGS 318



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.323    0.136    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 5,575,417
Number of extensions: 185870
Number of successful extensions: 433
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 431
Number of HSP's successfully gapped: 1
Length of query: 326
Length of database: 11,106,569
Length adjustment: 99
Effective length of query: 227
Effective length of database: 8,392,385
Effective search space: 1905071395
Effective search space used: 1905071395
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 112 (47.8 bits)