BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0527500 Os04g0527500|AK059144
         (124 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT4G15010.1  | chr4:8573125-8574864 REVERSE LENGTH=379            140   1e-34

>AT4G15010.1 | chr4:8573125-8574864 REVERSE LENGTH=379
          Length = 378

 Score =  140 bits (354), Expect = 1e-34,   Method: Compositional matrix adjust.
 Identities = 63/124 (50%), Positives = 79/124 (63%)

Query: 1   HTAMLTWSAVNMNPQPSKLEEAGPVSPXXXXXXXXXXXXXXXXXXHTFDTAKSRSECTVI 60
           H AM+ W AV MNP PS  EE GP+SP                  H+FDTA++R++C ++
Sbjct: 253 HEAMVGWKAVGMNPLPSSEEEVGPLSPVAISLAAGISGAVAAAASHSFDTARTRAQCVIL 312

Query: 61  PKYIAMERRLLKWKVPGNWIERKTGISPADRNVLFRGIGQRMARSGFASFLLVGSYYFVI 120
           PKY A ER+ LKW  PG  +ER TGI P DRN+LFRGIG RMARS  AS ++VGSYY  +
Sbjct: 313 PKYTAKERKFLKWNKPGKRLERWTGIHPTDRNLLFRGIGMRMARSSVASTVIVGSYYLAV 372

Query: 121 DQFL 124
           D  +
Sbjct: 373 DLLV 376



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.322    0.135    0.423 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 2,377,561
Number of extensions: 73393
Number of successful extensions: 100
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 99
Number of HSP's successfully gapped: 1
Length of query: 124
Length of database: 11,106,569
Length adjustment: 86
Effective length of query: 38
Effective length of database: 8,748,793
Effective search space: 332454134
Effective search space used: 332454134
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 105 (45.1 bits)