BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0525200 Os04g0525200|AK121009
         (208 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G22250.1  | chr3:7867806-7870053 FORWARD LENGTH=462            112   2e-25
AT3G02100.1  | chr3:368840-370484 REVERSE LENGTH=465               75   3e-14

>AT3G22250.1 | chr3:7867806-7870053 FORWARD LENGTH=462
          Length = 461

 Score =  112 bits (279), Expect = 2e-25,   Method: Compositional matrix adjust.
 Identities = 70/177 (39%), Positives = 96/177 (54%), Gaps = 23/177 (12%)

Query: 6   IQQQPAVLLVPFPAQGHVTPMLNLXXXXXXXXXXXXXXXPDFIHXXXXXXXXXXXXXXDN 65
           + Q+P ++ +P+PAQGHVTPML+L               P+ IH               N
Sbjct: 3   VTQKPKIIFIPYPAQGHVTPMLHLASAFLSRGFSPVVMTPESIHRRISAT---------N 53

Query: 66  QAVGGGVELASIPSGIPHLPAGESGGGRHADDQPGFGAIVHAMEHHMPEQLERMLLSTAG 125
           + +G      S     P  P  +            F +I ++ME+ MP QLER+LL    
Sbjct: 54  EDLGITFLALSDGQDRPDAPPSD------------FFSIENSMENIMPPQLERLLLEEDL 101

Query: 126 RGRVACLVVDVLASWAVPVAERCGVPAAGFWPAMLASYRAVAAIPELLRKGVISESG 182
              VAC+VVD+LASWA+ VA+RCGVP AGFWP M A+YR + AIPEL+R G++S+ G
Sbjct: 102 D--VACVVVDLLASWAIGVADRCGVPVAGFWPVMFAAYRLIQAIPELVRTGLVSQKG 156



>AT3G02100.1 | chr3:368840-370484 REVERSE LENGTH=465
          Length = 464

 Score = 75.1 bits (183), Expect = 3e-14,   Method: Compositional matrix adjust.
 Identities = 47/180 (26%), Positives = 82/180 (45%), Gaps = 9/180 (5%)

Query: 9   QPAVLLVPFPAQGHVTPMLNLXXXXXXXXXXXXXXXPDFIHXXXXXXXXXXXXXXDNQAV 68
           +P V+++P+PAQGHV P+++                 +F H                  V
Sbjct: 11  RPHVVVIPYPAQGHVLPLISFSRYLAKQGIQITFINTEFNHNRIISSLPNSPH---EDYV 67

Query: 69  GGGVELASIPSGIPHLPAGESGGGRHADDQPGFGAIVHAMEHHMPEQLERMLLSTAGRGR 128
           G  + L SIP G+   P   +  G+ ++      +++  M   + E +ERM+  T+G   
Sbjct: 68  GDQINLVSIPDGLEDSPEERNIPGKLSE------SVLRFMPKKVEELIERMMAETSGGTI 121

Query: 129 VACLVVDVLASWAVPVAERCGVPAAGFWPAMLASYRAVAAIPELLRKGVISESGTIDIDT 188
           ++C+V D    WA+ VA + G+    F PA  AS     +I +L+  G+I   GT+ ++ 
Sbjct: 122 ISCVVADQSLGWAIEVAAKFGIRRTAFCPAAAASMVLGFSIQKLIDDGLIDSDGTVRVNK 181



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.321    0.137    0.428 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,935,379
Number of extensions: 142200
Number of successful extensions: 394
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 393
Number of HSP's successfully gapped: 2
Length of query: 208
Length of database: 11,106,569
Length adjustment: 94
Effective length of query: 114
Effective length of database: 8,529,465
Effective search space: 972359010
Effective search space used: 972359010
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 109 (46.6 bits)