BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0512300 Os04g0512300|AK071791
         (368 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G33385.2  | chr2:14147604-14149769 REVERSE LENGTH=375          243   1e-64
AT1G30825.1  | chr1:10961106-10962999 FORWARD LENGTH=319          179   2e-45

>AT2G33385.2 | chr2:14147604-14149769 REVERSE LENGTH=375
          Length = 374

 Score =  243 bits (619), Expect = 1e-64,   Method: Compositional matrix adjust.
 Identities = 123/326 (37%), Positives = 190/326 (58%), Gaps = 10/326 (3%)

Query: 1   MAFFSSGSRALVEILTGLQSVERPMPVDHTLFEFGSIRYHLQASITDSENIYLSISTPSL 60
           MA+    S  L E L  +   E+P+ VD    EFGSI YH++ S++D   +++S ST   
Sbjct: 1   MAYLERASPTLKETLLKIYRAEKPIEVDQHFHEFGSIEYHIKYSVSDPNIVHVSTSTLLE 60

Query: 61  SYEASPSSGLPEITLQETRKMYHKFAEIIEPAKEGYTLTLRLNFSGLTRPKDRTKAXXXX 120
           +  A     +   T +  + +     +I++P + G+ LTL LN   + R K+  K     
Sbjct: 61  TQGAVTLKEISSYTYEVIKNIGVGVIDIVDPPRLGFQLTLGLNLDNIPRGKEAIKIITRI 120

Query: 121 XXXXXXXXXXXXKDMLASLG--------SSGTMKLVYNQRDPFFVSKTPVKISAIFPMRF 172
                       K+ML SL         ++  +++VY+  +PF+V K P KI+A+FPM F
Sbjct: 121 SELQAIILSSQLKEMLRSLNFQDDSRSINNMPIRIVYHPSEPFYVFKQPEKITAVFPMNF 180

Query: 173 RDDTDLAIASSFFQELQDLGSTSSFSRAPRCSWSPIPPPELRGEYVHHLTTNGGFVSFDI 232
           +D++D+ IA+SFFQEL ++GS     +AP+CSWSPIPP +LRGE V  LTTN GFVSFDI
Sbjct: 181 KDNSDVVIATSFFQELVEVGSQKDMGKAPQCSWSPIPPLQLRGEPVQDLTTNSGFVSFDI 240

Query: 233 LARHVKGRRAARTAWILLNFQSYVKYHIKCTRSYIQSRMRKRLEIMTEVIDDAKFRGNDE 292
            +RH++G+R  +T W LLNF +  KYHIKC+R YIQ RMRKR+E + +++++      +E
Sbjct: 241 TSRHIEGKRLDKTVWNLLNFYACAKYHIKCSRGYIQRRMRKRMETLVKLLNNTSLE--EE 298

Query: 293 SSKKLQVRKRSKRRSIKFARAKKLQK 318
           +++    R +  +  +K  + K + K
Sbjct: 299 AAQNENGRCKYVKEFVKVPKGKLMMK 324



>AT1G30825.1 | chr1:10961106-10962999 FORWARD LENGTH=319
          Length = 318

 Score =  179 bits (455), Expect = 2e-45,   Method: Compositional matrix adjust.
 Identities = 98/292 (33%), Positives = 159/292 (54%), Gaps = 7/292 (2%)

Query: 1   MAFFSSGSRALVE-ILTGLQSVERPMPVDHTLFEFGSIRYHLQASITDSENIYLSISTPS 59
           M    S SR L++ +LT  Q++++ + +D+   EF  +RYH+Q ++ +   + LS+S P+
Sbjct: 1   MILLQSHSRFLLQTLLTRAQNLDKAVELDYQWIEFDDVRYHVQVTMKNPNLLLLSVSLPN 60

Query: 60  LSYEASPSSGLPEITLQETRKMYHKFAEIIEPAKEGYTLTLRLNFSGLTRPKDR--TKAX 117
              EA    GLP   ++  +  Y    +I++P ++G++LTL+LNFS + RP +   TK  
Sbjct: 61  PPPEAMSFDGLPLGAIEAIKTTYGTGFQILDPPRDGFSLTLKLNFSKV-RPDEELLTKLA 119

Query: 118 XXXXXXXXXXXXXXXKDMLASLGSSGTMKLVYNQRDP---FFVSKTPVKISAIFPMRFRD 174
                          K + +   +    +LV     P   FF+     K++  FPMRF+D
Sbjct: 120 SIREVVMGAPLKIIFKHLASRTVAPELDRLVAIMHRPNETFFLVPQADKVTVAFPMRFKD 179

Query: 175 DTDLAIASSFFQELQDLGSTSSFSRAPRCSWSPIPPPELRGEYVHHLTTNGGFVSFDILA 234
             D  +A+SF +E  +    ++ + AP CSWSP  P EL G     L+ N GFV+F I  
Sbjct: 180 SVDTILATSFLKEFVEARRAAALNTAPSCSWSPTAPQELEGAPKETLSANAGFVTFVIFP 239

Query: 235 RHVKGRRAARTAWILLNFQSYVKYHIKCTRSYIQSRMRKRLEIMTEVIDDAK 286
           RHV+G++  RT W L  F +YV YH+K +  ++ +RMR+R+E M + +D AK
Sbjct: 240 RHVEGKKLDRTVWNLSTFHAYVSYHVKFSEGFMHTRMRRRVESMIQALDQAK 291



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.321    0.134    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 7,129,971
Number of extensions: 279017
Number of successful extensions: 688
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 685
Number of HSP's successfully gapped: 2
Length of query: 368
Length of database: 11,106,569
Length adjustment: 100
Effective length of query: 268
Effective length of database: 8,364,969
Effective search space: 2241811692
Effective search space used: 2241811692
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 112 (47.8 bits)