BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os04g0498900 Os04g0498900|J065071K10
(190 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT4G31400.1 | chr4:15237411-15239266 FORWARD LENGTH=346 142 1e-34
>AT4G31400.1 | chr4:15237411-15239266 FORWARD LENGTH=346
Length = 345
Score = 142 bits (358), Expect = 1e-34, Method: Compositional matrix adjust.
Identities = 83/173 (47%), Positives = 104/173 (60%), Gaps = 9/173 (5%)
Query: 17 FGEGQLLHKLCKVYLFISSQRIVGCLVAEPIKTAHKVIPGSTEENGTDLPVDKIEPVKTN 76
GE +LH+ CKVYLFISSQRI GCLVAEPIK A K+I +E + K +
Sbjct: 174 LGEDWILHQHCKVYLFISSQRISGCLVAEPIKEAFKLIASPDDER----QLQKESSSSPS 229
Query: 77 HTLEFGKISFKREVLKRHDHSDKNKEEYRDPGAIICEKEDVPAHCGFRAIWVVPSRRRKR 136
+++FG I +REV KR SD + D G I+CE+E PA CG RAIWV PS RRK
Sbjct: 230 TSIQFGNIVLQREVSKRCRTSD----DRLDNGVIVCEEEAKPAVCGIRAIWVSPSNRRKG 285
Query: 137 IGSQLMDAARKSFL-EGETLCISQCAFSPPTSSGKALARSYCKTSAFLVYKEQ 188
I + L+D R+SF G L SQ AFS P+S G++ Y T +FL+YK Q
Sbjct: 286 IATWLLDTTRESFCNNGCMLEKSQLAFSQPSSIGRSFGSKYFGTCSFLLYKAQ 338
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.320 0.136 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,245,194
Number of extensions: 179763
Number of successful extensions: 385
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 382
Number of HSP's successfully gapped: 1
Length of query: 190
Length of database: 11,106,569
Length adjustment: 93
Effective length of query: 97
Effective length of database: 8,556,881
Effective search space: 830017457
Effective search space used: 830017457
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 109 (46.6 bits)