BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0485200 Os04g0485200|AK069013
         (298 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G27310.1  | chr3:10087360-10089077 REVERSE LENGTH=252          191   3e-49
AT2G01650.1  | chr2:284851-286394 REVERSE LENGTH=459               55   5e-08

>AT3G27310.1 | chr3:10087360-10089077 REVERSE LENGTH=252
          Length = 251

 Score =  191 bits (486), Expect = 3e-49,   Method: Compositional matrix adjust.
 Identities = 100/203 (49%), Positives = 140/203 (68%), Gaps = 9/203 (4%)

Query: 64  TTSQDKLKALAYEYGHEFRVFSSVTFESMTSNLPAADQEEDDDFYELQPADYFNLVSNRI 123
           +++Q K+  +  + G E RVF + +     S + +AD +E DDFYE  PAD++ L++ + 
Sbjct: 26  SSAQAKIADMREKLGREVRVFETSSISQRPSQVSSAD-DESDDFYEFTPADFYRLLATK- 83

Query: 124 GEQSKVLKTRKMREAELAAQRAKIKKAVMRVRFPDGYILEADFHPSETVQSLMDFLKKVI 183
            ++ K LKTRK+REAE AA+R+K+ KAV+RVRFPD + LEA FHPSE +Q L+D +K+V+
Sbjct: 84  -KEDKSLKTRKIREAEEAARRSKLTKAVIRVRFPDNHTLEATFHPSEKIQGLIDLVKRVV 142

Query: 184 SRPDLPFYLYTVPPKKRIKDTSLDFYTIGFVPGANVYFSYDLPEGSELNTDSVKSGPYLR 243
           + PD+PFYLYT PPKK+IKD S DFY+ GFVPGA VYFS D P+      D   S PYL 
Sbjct: 143 AHPDVPFYLYTTPPKKQIKDFSQDFYSAGFVPGAIVYFSNDQPKD-----DGGSSTPYLN 197

Query: 244 EEIRMLDGLPIVQEPVHQPIDST 266
           EEI  L  L  + + V +P++S+
Sbjct: 198 EEILSLKDLEAMTKAV-EPVESS 219



>AT2G01650.1 | chr2:284851-286394 REVERSE LENGTH=459
          Length = 458

 Score = 55.1 bits (131), Expect = 5e-08,   Method: Compositional matrix adjust.
 Identities = 41/118 (34%), Positives = 63/118 (53%), Gaps = 12/118 (10%)

Query: 79  HEFRVFSSVTFESMTSNLPAADQEEDDDFYELQPADYF----NLVSNRIGEQSKVLKTRK 134
            E RVF SV+ E++ S +     E  D FY L  AD      +L   +I E S++L  R 
Sbjct: 287 REIRVFFSVS-ENVASRI-----EVPDSFYSLS-ADEIKREADLRRKKIAE-SQLLIPRS 338

Query: 135 MREAELAAQRAKIKKAVMRVRFPDGYILEADFHPSETVQSLMDFLKKVISRPDLPFYL 192
            +E +  A R + K++++RV+FPDG +L+  F P E   +L +F+   +  P L F L
Sbjct: 339 YKEKQAKAARKRYKRSMIRVQFPDGVVLQGVFAPWEPTFALYEFVSSALKEPSLQFEL 396



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.319    0.136    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 5,257,663
Number of extensions: 201830
Number of successful extensions: 540
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 537
Number of HSP's successfully gapped: 2
Length of query: 298
Length of database: 11,106,569
Length adjustment: 98
Effective length of query: 200
Effective length of database: 8,419,801
Effective search space: 1683960200
Effective search space used: 1683960200
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 111 (47.4 bits)