BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0463400 Os04g0463400|AK059730
         (252 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G01770.1  | chr2:334775-336425 REVERSE LENGTH=251              305   1e-83

>AT2G01770.1 | chr2:334775-336425 REVERSE LENGTH=251
          Length = 250

 Score =  305 bits (782), Expect = 1e-83,   Method: Compositional matrix adjust.
 Identities = 141/205 (68%), Positives = 167/205 (81%)

Query: 27  ERHFTSGEVVRDVIMGVSDGLTVPFXXXXXXXXXXXXXXXXXTAGLAEVAAGAISMGLGG 86
           E+HFT+GE+VRD+I+GVSDGLTVPF                 TAG+AEVAAGAISMGLGG
Sbjct: 26  EKHFTAGEIVRDIIIGVSDGLTVPFALAAGLSGANASSSIVLTAGIAEVAAGAISMGLGG 85

Query: 87  YLAAKSEADHYQREMKREQEEIIAVPDTEAAEIGEIMSQYGLEPHEYGPVVDGLRRNPQA 146
           YLAAKSE DHY REMKREQEEI+AVP+TEAAE+ EI++QYG+EPHEY PVV+ LR+NPQA
Sbjct: 86  YLAAKSEEDHYAREMKREQEEIVAVPETEAAEVAEILAQYGIEPHEYSPVVNALRKNPQA 145

Query: 147 WLDFMMRFELGLEKPDPKRAIQSALTIALSYVIGGLVPLLPYMFISTAQNAMLTSVGVTL 206
           WLDFMMRFELGLEKPDPKRA+QSA TIA++YV+GG +PLLPYM I  A +A++ SV +TL
Sbjct: 146 WLDFMMRFELGLEKPDPKRALQSAFTIAIAYVLGGFIPLLPYMLIPHAMDAVVASVVITL 205

Query: 207 VALLFFGYIKGRFTGNRPFLSAVQT 231
            AL  FGY KG FTG++P  SA +T
Sbjct: 206 FALFIFGYAKGHFTGSKPLRSAFET 230



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.320    0.137    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,103,555
Number of extensions: 142636
Number of successful extensions: 397
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 398
Number of HSP's successfully gapped: 1
Length of query: 252
Length of database: 11,106,569
Length adjustment: 96
Effective length of query: 156
Effective length of database: 8,474,633
Effective search space: 1322042748
Effective search space used: 1322042748
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 110 (47.0 bits)