BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0462300 Os04g0462300|AK109651
         (340 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G25805.1  | chr3:9426354-9428169 REVERSE LENGTH=336            333   9e-92

>AT3G25805.1 | chr3:9426354-9428169 REVERSE LENGTH=336
          Length = 335

 Score =  333 bits (853), Expect = 9e-92,   Method: Compositional matrix adjust.
 Identities = 154/252 (61%), Positives = 190/252 (75%)

Query: 89  RALLRATLWGAEAAYILWLFLLPYAPGDPVWGISQATISDLIGXXXXXXXXXXXXXXAGV 148
           R ++R  LW +EA YILWLFLLPYAPGDPVW IS  T++ L+G              AG+
Sbjct: 82  RRIVRYLLWTSEAVYILWLFLLPYAPGDPVWAISSETVNSLLGLSLNFFFVLPLTNAAGI 141

Query: 149 HLLESPVLHPVAEGLFNFVIAWTLLFAPLLFTDSRRDRFKGSLDLLWGFQMFLTNTFLIP 208
           H L++PVLHP+AEGLFNFVIAWTL+FAPLL+TD +R+R+K SLD+LW   MFLTNTFLIP
Sbjct: 142 HFLQAPVLHPMAEGLFNFVIAWTLMFAPLLYTDRKRNRYKSSLDVLWSLMMFLTNTFLIP 201

Query: 209 YMAIRLNDPDKDKSPPQTSKLGSVMVRGAPIVGLTGGLVCILSIAWALFGRADASFGGIA 268
           YMA+RLND D D  P + S+LG VM +GAPIVGLTG  VC++S  W+L+GRAD  FGGI 
Sbjct: 202 YMALRLNDADPDDKPSKRSQLGEVMTKGAPIVGLTGATVCLISALWSLYGRADGDFGGIM 261

Query: 269 ERWQYVQSYVFSERLAYAFLWDILLYSIFQPWLIADNIQNVKASATEFVNSVRFLPVIGL 328
           +RWQY+  Y+ SERLAYAF+WDI LY+IFQPWLI +N+QNVK +  E V  +RF+PV GL
Sbjct: 262 DRWQYLIGYLGSERLAYAFIWDICLYTIFQPWLIGENLQNVKKNKIELVKYLRFVPVFGL 321

Query: 329 VAYLFCLEDRDD 340
           +AY   L   D+
Sbjct: 322 LAYHLFLNLEDE 333



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.327    0.141    0.456 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 5,735,705
Number of extensions: 206985
Number of successful extensions: 474
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 474
Number of HSP's successfully gapped: 1
Length of query: 340
Length of database: 11,106,569
Length adjustment: 99
Effective length of query: 241
Effective length of database: 8,392,385
Effective search space: 2022564785
Effective search space used: 2022564785
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 112 (47.8 bits)