BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os04g0418000 Os04g0418000|AK106155
(231 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT1G47420.1 | chr1:17395774-17397176 REVERSE LENGTH=258 213 6e-56
>AT1G47420.1 | chr1:17395774-17397176 REVERSE LENGTH=258
Length = 257
Score = 213 bits (542), Expect = 6e-56, Method: Compositional matrix adjust.
Identities = 100/171 (58%), Positives = 127/171 (74%)
Query: 61 RLFSSNEKHLPAISDPEVESAFKDLMAASWTGLPDSLVIEXXXXXXXXXXXXXXXEALLN 120
R FS + H+P + D +V +AFKDLMAA W LP ++V + EAL N
Sbjct: 87 RRFSEDVSHMPEMEDSDVLNAFKDLMAADWAELPSAVVKDAKTAISKNTDDKAGQEALKN 146
Query: 121 VFRAAEACEEFGGVLVTLRMALDDLCGITGENVGPLPGYIEDAVKSAYKRYMKYLESFGP 180
VFRAAEA EEFGG+L +++M +DD G++GE V PLP I DA+++AY+RY +YL+SF P
Sbjct: 147 VFRAAEAVEEFGGILTSIKMEIDDSIGMSGEGVKPLPNDITDALRTAYQRYAEYLDSFEP 206
Query: 181 EENYLRKKVENELGTKMIHLKMRCSGVGSEWGKITLIGTSGISGSYVELRA 231
EE YL+KKVE ELGTKMIHLKMRCSG+GSEWGK+T++GTSG+SGSYVE RA
Sbjct: 207 EEVYLKKKVEMELGTKMIHLKMRCSGLGSEWGKVTVLGTSGLSGSYVEQRA 257
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.317 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,722,197
Number of extensions: 135879
Number of successful extensions: 319
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 318
Number of HSP's successfully gapped: 1
Length of query: 231
Length of database: 11,106,569
Length adjustment: 95
Effective length of query: 136
Effective length of database: 8,502,049
Effective search space: 1156278664
Effective search space used: 1156278664
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 110 (47.0 bits)