BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0401300 Os04g0401300|AK121122
         (370 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G09020.1  | chr1:2900149-2904212 REVERSE LENGTH=488            287   5e-78

>AT1G09020.1 | chr1:2900149-2904212 REVERSE LENGTH=488
          Length = 487

 Score =  287 bits (735), Expect = 5e-78,   Method: Compositional matrix adjust.
 Identities = 156/361 (43%), Positives = 225/361 (62%), Gaps = 12/361 (3%)

Query: 21  YLKQMPPDQ--LSQNSGVQIAIFRHRVSEILLHNTIYDVVPVSSKIAVLDARLPVKQAFK 78
           +L+   P Q  + + SGV + + RHR+S +L   T Y+++P S K+  LD  LPVKQAF 
Sbjct: 127 FLRTADPSQEAVPRMSGVDLELSRHRISVLLSTRTAYELLPESGKVIALDVNLPVKQAFH 186

Query: 79  IMHDEGLSLVPLWDDQQQTVTGMLTASDFVLILRKLQRNIRTLGHEELEMHSVSAWKEAK 138
           I++++G+ L PLWD  +    G+L   DF+LILR+L  +   L  EELE H+++AWKE K
Sbjct: 187 ILYEQGIPLAPLWDFGKGQFVGVLGPLDFILILRELGTHGSNLTEEELETHTIAAWKEGK 246

Query: 139 LQFYGGPDVAAIQR-RPLIHVKDSDNLRDVALAIIRNEISSVPIFKPSTDSSGMP-LLGL 196
                  D +     RPL+ V   DNL+DVAL I++N++++VP+   S      P LL L
Sbjct: 247 AHISRQYDGSGRPYPRPLVQVGPYDNLKDVALKILQNKVAAVPVIYSSLQDGSYPQLLHL 306

Query: 197 ATLPGIVKFICSKLQEQPEGYSFLQNQIVSMPIGTWSPHTGKASNRQLRTSRPSTPLNSC 256
           A+L GI+K IC   +        LQ  I S+P+GTW P  G++S++ L T RP   L S 
Sbjct: 307 ASLSGILKCICRYFRHSSSSLPILQQPICSIPLGTWVPRIGESSSKPLATLRPHASLGSA 366

Query: 257 LDLLLEDRVSSIPIVDDNGALLDVYSLSDIMALGKNDVYTRIELEQVTVEHALELQYQV- 315
           L LL++  VSSIP+VDDN +L+D+YS SDI AL K+  Y +I L+ +TV  AL+L     
Sbjct: 367 LALLVQAEVSSIPVVDDNDSLIDIYSRSDITALAKDKAYAQIHLDDMTVHQALQLGQDAS 426

Query: 316 ------NGRRHCHTCLSTSTFLEVLEQLSAPGVRRVVVIEPRSRFVQGIISLRDAFTFLI 369
                 NG+R CH CL + + ++V+E+L+ PGVRR+V++E  S+ V+GIISL D F FL+
Sbjct: 427 PPYGIFNGQR-CHMCLRSDSLVKVMERLANPGVRRLVIVEAGSKRVEGIISLSDVFQFLL 485

Query: 370 G 370
           G
Sbjct: 486 G 486



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.322    0.137    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 7,681,793
Number of extensions: 311666
Number of successful extensions: 718
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 714
Number of HSP's successfully gapped: 1
Length of query: 370
Length of database: 11,106,569
Length adjustment: 100
Effective length of query: 270
Effective length of database: 8,364,969
Effective search space: 2258541630
Effective search space used: 2258541630
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 112 (47.8 bits)