BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0394300 Os04g0394300|AK111819
         (917 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G27700.1  | chr3:10257681-10261330 REVERSE LENGTH=909          416   e-116

>AT3G27700.1 | chr3:10257681-10261330 REVERSE LENGTH=909
          Length = 908

 Score =  416 bits (1068), Expect = e-116,   Method: Compositional matrix adjust.
 Identities = 288/812 (35%), Positives = 409/812 (50%), Gaps = 84/812 (10%)

Query: 62  RNHKHR-KREARPQSFDENT-EQSPGGPLKKRHKISGGADSHGEAQKDFFPKFKRRPGAG 119
           RNHKHR K E R QS ++++ +Q+   P +K ++       H E    F    KR  G G
Sbjct: 36  RNHKHRRKEETRSQSLEQDSSDQAFSRPYRKNYR-------HYENGNSFSEHEKRSFGTG 88

Query: 120 AHSRAP------RVNPSF-RDSSASVAARAPMTRGRGRNGAPWAQHEPRFNTLEMIDFAS 172
           +  R        R NP F RDS           RGRG  G+ WAQ + RFN +++     
Sbjct: 89  SGQRVQFDNQRMRSNPMFSRDSGPG--------RGRGNYGS-WAQRDSRFNPVDLSSHMV 139

Query: 173 QMASQGPPTHPSLFMGPALPSGGSAQNGSWGPYGFMPGMPNGMLDPIHPLGMQGPIQPAI 232
           Q+ S G      +F G  L    +AQ+  W P+G +PG+PNG LD  H L  QG ++P +
Sbjct: 140 QVGSMGQ----GMFGGRGLAGVSAAQSAPWPPFGMIPGVPNGGLDGFHHL--QGSLRPPL 193

Query: 233 SPLIDLGMPRQRCRDFEERGFCLRGDMCPMEHGLNRIVVEDMQSLSQFNLPVTVPNTQGL 292
           +  +++G+PRQRCRDFEERGFCLRGDMCPMEHG+NRIVV+D+QSLSQFNLPV+VP    L
Sbjct: 194 NAPLNMGIPRQRCRDFEERGFCLRGDMCPMEHGMNRIVVDDVQSLSQFNLPVSVPGAPHL 253

Query: 293 GIQNEPGTAPVNTSSLGGSKGVPAKDIKSAVTNDVLKLNGTTALAVSDADVYDPDQPLWN 352
              ++   A    +S    KG   +  +  +  D L   G    +    D YDPDQPLWN
Sbjct: 254 AASSKQVPAQFGGASFMNPKGAHGRTNEGGMAVDGLGY-GDAYPSAGGTDFYDPDQPLWN 312

Query: 353 NEHPDASAGFAHTDGVWNAESLGYEAAREQGNQVLAAD-----SSQNSKSSVWGRIASKK 407
           N   + S   +  +     E++   A  +  NQ  A +      S+++  SVWGR+   K
Sbjct: 313 NSTGETSGAISTLNSHGVDENV---APLDDSNQDAAENGCGIRDSRSTSQSVWGRM---K 366

Query: 408 LGHXXXXXXXXXXXXXXXRNESYDEMAPSTVHVNPASAKDSNGQS------NSRIFGDVG 461
             +                    D++   +V+ +    ++  G+S      +S I  D+ 
Sbjct: 367 GSNSQANSKEKADAVLNSSAVPEDQLKEVSVNSSRHGKQNHVGESVAKVVDSSNISNDMM 426

Query: 462 RQSNRASHKASRTLYVNGIPLESNRWEALLSHFQKFGQVIDIYIPSNSEKAFVQFSKREE 521
             + + + KA RTL+VN +P ESNR + +L+HFQKFG+VIDI+IP NSE+AFVQFSKREE
Sbjct: 427 NNTRKPTQKAMRTLFVNYVPHESNRRDLILAHFQKFGKVIDIHIPVNSERAFVQFSKREE 486

Query: 522 AEAALKAPDAVMGNRFIKLWWANRDRIPDE---VEGRIPAKSSHMSAALA-NSVPQPSSS 577
           AE+AL+APDAVMGNRFIKLWWANRD IPD           K   M+A+ A N  P  ++S
Sbjct: 487 AESALRAPDAVMGNRFIKLWWANRDSIPDNGLSTGSGASMKGRSMTASGAQNQFPIAAAS 546

Query: 578 NRGKEN-------LQSATPRXXXXXXXXXXXXXTGHKMLPANSVKSLPPDTXXXXXXXXX 630
                +        Q+                 +G K+ P    K+   DT         
Sbjct: 547 KSNHVSSIAKVPTFQTGGAPSSSEQPKPVVVTTSGPKVTPLQQKKA---DTLERLKETLR 603

Query: 631 XXXXXXXXXXAQKRDEFRRQLEKLAKQKGLANSAKQAEAGGKEVASNDVHRVTDSKSMNT 690
                      QKR+E+R++L  L KQ  +    +  E   K V      ++  +     
Sbjct: 604 KKQEMLE----QKRNEYRKKLATLEKQGTVVKREEADEPDAKRV------KLDTASDSGA 653

Query: 691 GTEGPRDAAGTLQN-------RTXXXXXXXXXXXXXXXXQKPAVATKQTSPLLVPSQNRF 743
               P+  + T +         T                Q+P      T+ L  P  NR+
Sbjct: 654 AIASPKTESSTDKKVPILKPLSTAKLSTETPSPDSKNFKQRPYSF---TTSLNAPMVNRY 710

Query: 744 KLDNRTTSFRILPPLPPEIADESVLKDHFMSFGELSSVVLEDTEAYNHDATLKP-SLSCS 802
           KLDNRTT+ +++PPLP  +AD +V+K+HF S+GE+S V LED  + +     +  + S +
Sbjct: 711 KLDNRTTTIKVVPPLPTGLADVAVVKEHFSSYGEVSKVELEDNASIDSGKDHETQNESRA 770

Query: 803 ACVTYTTRQSAEKAFIGGKSCKGHTLRFMWLT 834
           ACVT+  R +AEKAF   K  + HTL+ +W+T
Sbjct: 771 ACVTFVKRSAAEKAFANAKCWQEHTLQLVWVT 802



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.310    0.126    0.365 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 19,002,936
Number of extensions: 803032
Number of successful extensions: 2170
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 2172
Number of HSP's successfully gapped: 2
Length of query: 917
Length of database: 11,106,569
Length adjustment: 108
Effective length of query: 809
Effective length of database: 8,145,641
Effective search space: 6589823569
Effective search space used: 6589823569
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 116 (49.3 bits)