BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0391900 Os04g0391900|AK101777
         (369 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G35450.1  | chr2:14903201-14905361 REVERSE LENGTH=347          428   e-120

>AT2G35450.1 | chr2:14903201-14905361 REVERSE LENGTH=347
          Length = 346

 Score =  428 bits (1100), Expect = e-120,   Method: Compositional matrix adjust.
 Identities = 193/284 (67%), Positives = 239/284 (84%), Gaps = 1/284 (0%)

Query: 84  VVDSHLHVWASPQQAAERYPYFPGQEPPIRGDVDLLLQCMDEAGVDGALIVQPINHMFDH 143
           V+DSHLH+WASPQ+A E YPYFPGQEP + GDV+ LL+ M+EA VDGALIVQPINH FDH
Sbjct: 62  VIDSHLHIWASPQEA-ETYPYFPGQEPTLTGDVNFLLKNMEEARVDGALIVQPINHKFDH 120

Query: 144 SLVTSVLKKYPSKFIGCCLANPADDGSGIKQLEHLIVQEKYRAVRFNPNLWPSGQKMTNE 203
           SLVTSVLK+YPSKF+GCCLANPA+DGSGI  LE+L+++  YRAVRFNP LWPSGQKMTN 
Sbjct: 121 SLVTSVLKRYPSKFVGCCLANPAEDGSGITHLENLVLESNYRAVRFNPYLWPSGQKMTNA 180

Query: 204 VGRSLFAKAGELGAPVGIMVMKGISTYIQEIEELCTDYPKTTVIFDHMAFCKPPMNIEEE 263
           VG++LF+KAGEL  PVG M MKG+  +I EIEELCT++PKTTV+ DH  FCK P + E +
Sbjct: 181 VGKALFSKAGELCVPVGFMCMKGLDLHIAEIEELCTEFPKTTVLLDHAGFCKVPESGEAK 240

Query: 264 KAFTSFLELSRFPQIYVKYSALFRISREAYPYEDTSQLLSRVISSYGANRIMWGSDFPFV 323
            A++  ++LSRFPQ+YVK+SALFRISR  +PY+D S LLS+++S +GANR+MWGSDFPFV
Sbjct: 241 LAYSQLMKLSRFPQVYVKFSALFRISRTGFPYQDLSPLLSQLVSHFGANRVMWGSDFPFV 300

Query: 324 VPECGYKGAKEAISHVAGKIPVSSSDLEWILGKTVTQLFQGAWV 367
           V ECGYK AKEA++ +A +  +SSS+++WILGKT+ QLF G WV
Sbjct: 301 VLECGYKEAKEAVTIIAKEASLSSSEMDWILGKTLMQLFPGQWV 344



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.321    0.136    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 7,367,364
Number of extensions: 289911
Number of successful extensions: 736
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 735
Number of HSP's successfully gapped: 1
Length of query: 369
Length of database: 11,106,569
Length adjustment: 100
Effective length of query: 269
Effective length of database: 8,364,969
Effective search space: 2250176661
Effective search space used: 2250176661
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 112 (47.8 bits)