BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os04g0347200 Os04g0347200|J033091K14
(153 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT5G24090.1 | chr5:8143805-8145153 REVERSE LENGTH=303 130 2e-31
>AT5G24090.1 | chr5:8143805-8145153 REVERSE LENGTH=303
Length = 302
Score = 130 bits (328), Expect = 2e-31, Method: Compositional matrix adjust.
Identities = 64/138 (46%), Positives = 82/138 (59%), Gaps = 3/138 (2%)
Query: 16 RYLKAYSGRKPGGKKVWLTAAPQCPFPDRMLGEALRTGVFDRVHVQFYNNPXXXXXXXXX 75
R L +S R G+K++LT APQCPFPDR++G AL T FD V +QFYNNP
Sbjct: 168 RTLSKFSHR---GRKIYLTGAPQCPFPDRLMGSALNTKRFDYVWIQFYNNPPCSYSSGNT 224
Query: 76 XXXXXXXXXXXXXLPRSSVYLGLPAAPGAANSGYVPPAALAGEALPIVQRSRNYGGVMLW 135
+ +LGLPAAP AA SGY+PP L + LP +++SR YGGVMLW
Sbjct: 225 QNLFDSWNKWTTSIAAQKFFLGLPAAPEAAGSGYIPPDVLTSQILPTLKKSRKYGGVMLW 284
Query: 136 SRYWDRRTGYSKKIKHAV 153
S++WD + GYS I +V
Sbjct: 285 SKFWDDKNGYSSSILASV 302
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.322 0.139 0.455
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,089,829
Number of extensions: 102679
Number of successful extensions: 194
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 193
Number of HSP's successfully gapped: 1
Length of query: 153
Length of database: 11,106,569
Length adjustment: 90
Effective length of query: 63
Effective length of database: 8,639,129
Effective search space: 544265127
Effective search space used: 544265127
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 107 (45.8 bits)