BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0320100 Os04g0320100|AK068015
         (327 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G04620.1  | chr1:1282869-1286492 REVERSE LENGTH=463            501   e-142

>AT1G04620.1 | chr1:1282869-1286492 REVERSE LENGTH=463
          Length = 462

 Score =  501 bits (1290), Expect = e-142,   Method: Compositional matrix adjust.
 Identities = 232/272 (85%), Positives = 255/272 (93%), Gaps = 1/272 (0%)

Query: 51  LREDWRERSKAIPPGGVYPAKDHCSQCGLCDTYYIAHVKNACAFLGDGMSRVEDLEPLVH 110
           LREDWRE+S+ IPPGG YPAKDHCSQCGLCDTYYIAHVK ACAFLGDGMSR+E LEP+VH
Sbjct: 39  LREDWREKSRPIPPGGTYPAKDHCSQCGLCDTYYIAHVKEACAFLGDGMSRIESLEPVVH 98

Query: 111 GRGRKQD-MDEMYFGVYEQLLYARKMKPVEGAQWTGIVTTIAVEMLKANMVDAVVCVQSD 169
           GRGRK D + + YFGV+++ LYARK+KPVEGAQWTGIVTTIA+EMLK+NMV+AVVCVQSD
Sbjct: 99  GRGRKADSLQDTYFGVHQEQLYARKLKPVEGAQWTGIVTTIAIEMLKSNMVEAVVCVQSD 158

Query: 170 PDDRLAPMPVLARTPDEVIAAKGVKPTLSPNLNTLALVEAAGVKRLLFCGVGCQVQALRS 229
           P+DRL+P PVLARTP+EV+AA+GVKPTLSPNLNTL L+EA+GVKRLLFCGVGCQVQALRS
Sbjct: 159 PEDRLSPRPVLARTPEEVLAARGVKPTLSPNLNTLELIEASGVKRLLFCGVGCQVQALRS 218

Query: 230 VEKYLGLEKLYVLGTNCVDNGTREGLDKFLKAASSEPETVLHYEFMQDYKVHLKHLDGHI 289
           VE++L LEKLYVLGTNCVDNGTR+GLDKFLKAAS EPETVLHYEFMQDYKV LKHLDGHI
Sbjct: 219 VEQHLNLEKLYVLGTNCVDNGTRDGLDKFLKAASKEPETVLHYEFMQDYKVQLKHLDGHI 278

Query: 290 EEVPYFCLPAKDLVDVIAPSCYSCFDYTNGLA 321
           EEVPYF LPA DLVDVIAPSCYSCFDYTN LA
Sbjct: 279 EEVPYFSLPANDLVDVIAPSCYSCFDYTNALA 310



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.322    0.137    0.427 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 7,057,139
Number of extensions: 290046
Number of successful extensions: 604
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 603
Number of HSP's successfully gapped: 1
Length of query: 327
Length of database: 11,106,569
Length adjustment: 99
Effective length of query: 228
Effective length of database: 8,392,385
Effective search space: 1913463780
Effective search space used: 1913463780
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 112 (47.8 bits)