BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os04g0311100 Os04g0311100|AK059112
(63 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT4G24970.1 | chr4:12831125-12835449 FORWARD LENGTH=708 59 7e-10
AT5G50780.1 | chr5:20650269-20654948 FORWARD LENGTH=820 55 8e-09
>AT4G24970.1 | chr4:12831125-12835449 FORWARD LENGTH=708
Length = 707
Score = 58.5 bits (140), Expect = 7e-10, Method: Composition-based stats.
Identities = 30/51 (58%), Positives = 41/51 (80%)
Query: 4 AQRQLETSNKEQEALIDIFSEERGRRDLEEENLRGKLKDASSTIQDLLEQL 54
+++++E KEQE LI+IFSEER RRD EEE LR KL++AS+TI DLL ++
Sbjct: 644 SRQKIEEMKKEQENLIEIFSEERDRRDGEEEVLRNKLEEASNTIDDLLNKI 694
>AT5G50780.1 | chr5:20650269-20654948 FORWARD LENGTH=820
Length = 819
Score = 55.1 bits (131), Expect = 8e-09, Method: Compositional matrix adjust.
Identities = 28/52 (53%), Positives = 42/52 (80%)
Query: 1 LEDAQRQLETSNKEQEALIDIFSEERGRRDLEEENLRGKLKDASSTIQDLLE 52
+E + +L+ +KEQ +LID+F+E+R RRD EEENLR KL++AS+TIQ L++
Sbjct: 762 VETLKNKLKEMDKEQASLIDVFAEDRDRRDKEEENLRIKLEEASNTIQKLID 813
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.308 0.128 0.322
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 1,169,420
Number of extensions: 39980
Number of successful extensions: 264
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 264
Number of HSP's successfully gapped: 3
Length of query: 63
Length of database: 11,106,569
Length adjustment: 36
Effective length of query: 27
Effective length of database: 10,119,593
Effective search space: 273229011
Effective search space used: 273229011
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)
S2: 104 (44.7 bits)