BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0224600 Os04g0224600|AK058344
         (236 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT4G38370.1  | chr4:17970112-17971222 REVERSE LENGTH=226          257   4e-69

>AT4G38370.1 | chr4:17970112-17971222 REVERSE LENGTH=226
          Length = 225

 Score =  257 bits (657), Expect = 4e-69,   Method: Compositional matrix adjust.
 Identities = 126/220 (57%), Positives = 156/220 (70%), Gaps = 5/220 (2%)

Query: 18  NRYWILRHGRSVPNERGIIVSSLENGTKPEFGLAPQGVEQARLAGESLRKELEELGVPLD 77
           NRYW+LRHG+S+PNERG++VSS+ENG  PE+ LAP GV QARLAGES  ++L+E  + LD
Sbjct: 10  NRYWVLRHGKSIPNERGLVVSSMENGVLPEYQLAPDGVAQARLAGESFLQQLKESNIELD 69

Query: 78  SVQIRYSPFSRTMETAREVARVLGVPFDTPSCIPAVELRERYFGPSHELLSHEKYAEVWA 137
            V+I YSPFSRT  TAR VA+VL +PFD P C    +LRERYFGP+ EL SH+KY E+WA
Sbjct: 70  KVRICYSPFSRTTHTARVVAKVLNLPFDAPQCKMMEDLRERYFGPTFELKSHDKYPEIWA 129

Query: 138 VDEVDPLMAPDGGESVADVATRFSQFLSAAEMELHGSAILIVSHGDPLQIFQAVLKETKE 197
           +DE DP M P+GGES  DV +R +  + + E E    AIL+VSHGDPLQ+ Q V    K+
Sbjct: 130 LDEKDPFMGPEGGESADDVVSRLATAMKSMEAEYQRCAILVVSHGDPLQMLQNVFHSAKQ 189

Query: 198 NP-SSLDEVSDLKKRGMITLSVLSQHRQFALLTGELRRLV 236
                L E   L +      SVLSQHR+FALLTGELR L+
Sbjct: 190 QEGDGLAEKFQLSR----VASVLSQHRKFALLTGELRPLI 225



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.317    0.135    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,894,869
Number of extensions: 195421
Number of successful extensions: 508
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 508
Number of HSP's successfully gapped: 2
Length of query: 236
Length of database: 11,106,569
Length adjustment: 95
Effective length of query: 141
Effective length of database: 8,502,049
Effective search space: 1198788909
Effective search space used: 1198788909
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 110 (47.0 bits)