BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0136900 Os04g0136900|AK067888
         (354 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT4G38280.1  | chr4:17942034-17942864 FORWARD LENGTH=171           73   3e-13
AT2G45250.2  | chr2:18661464-18662781 FORWARD LENGTH=216           49   3e-06

>AT4G38280.1 | chr4:17942034-17942864 FORWARD LENGTH=171
          Length = 170

 Score = 72.8 bits (177), Expect = 3e-13,   Method: Compositional matrix adjust.
 Identities = 35/84 (41%), Positives = 54/84 (64%)

Query: 259 PGTTVQDDTHKSSNQYWNARFNRLQTYLESCDRSTQEGYMRMLRSLSAADRSMHAIDLEK 318
           P +  Q+ T  S    W  R+  LQ  L   ++S +  +++ML SLS+A+ S HA+DLEK
Sbjct: 76  PSSPAQEPTPTSHKLDWEERYLHLQMLLNKLNQSDRTDHVQMLWSLSSAELSKHAVDLEK 135

Query: 319 RAIHLLVEEGKELQRMKALNVLGK 342
           R+I   +EE +E+QR+ ALN+LG+
Sbjct: 136 RSIQFSLEEAREMQRVAALNMLGR 159



>AT2G45250.2 | chr2:18661464-18662781 FORWARD LENGTH=216
          Length = 215

 Score = 49.3 bits (116), Expect = 3e-06,   Method: Compositional matrix adjust.
 Identities = 23/53 (43%), Positives = 35/53 (66%)

Query: 275 WNARFNRLQTYLESCDRSTQEGYMRMLRSLSAADRSMHAIDLEKRAIHLLVEE 327
           W  R+  LQ  L   ++S +  +++ML SLS+A+ S HA+DLEKR+I   +EE
Sbjct: 126 WEERYLHLQMLLNKLNQSDRTDHVQMLWSLSSAELSKHAVDLEKRSIQFSLEE 178



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.303    0.119    0.326 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 8,067,715
Number of extensions: 364408
Number of successful extensions: 1296
Number of sequences better than 1.0e-05: 4
Number of HSP's gapped: 1285
Number of HSP's successfully gapped: 4
Length of query: 354
Length of database: 11,106,569
Length adjustment: 100
Effective length of query: 254
Effective length of database: 8,364,969
Effective search space: 2124702126
Effective search space used: 2124702126
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 43 (21.8 bits)
S2: 112 (47.8 bits)