BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0132300 Os04g0132300|AK121974
         (346 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT1G66510.1  | chr1:24812881-24815804 FORWARD LENGTH=400          359   1e-99

>AT1G66510.1 | chr1:24812881-24815804 FORWARD LENGTH=400
          Length = 399

 Score =  359 bits (921), Expect = 1e-99,   Method: Compositional matrix adjust.
 Identities = 175/349 (50%), Positives = 231/349 (66%), Gaps = 6/349 (1%)

Query: 1   MVPPGPHFLYYCSPNRHANEFAPTVGFFLTTHPSEVIVRKWHAQXXXXXXXXXXXXXXYS 60
           M+PPG HF++Y S  R   EF+PT+GFF+   PS+VIVRKW+ Q              YS
Sbjct: 45  MIPPGIHFVFYSSSTRDGREFSPTIGFFVDVAPSQVIVRKWNQQDEWLTKVSEEEEERYS 104

Query: 61  EAVRHFEFDSQLGPYNLDSFGDWKQLSSYLPQSVIERLEPIGGEITIAWESSWMDKAPQT 120
           +AVR  EFD  LGPYNL  +G+W+ LS+Y+ + V+E+ EP+GGEIT+ +ES+ +   P+T
Sbjct: 105 QAVRSLEFDKNLGPYNLKQYGEWRHLSNYITKDVVEKFEPVGGEITVTYESAILKGGPKT 164

Query: 121 DMERRLMDQLKDGKFAKNAPVQSERRGCYYTTIPASIKHSNISGDELTALNLDKTCLLES 180
            ME  L  Q+K  KF  ++  Q +    YYT+IP  IKH  +SG ELT++NLDKT LLES
Sbjct: 165 AMEIALDTQMKKSKFTTSSTEQPKGNRFYYTSIPRIIKHKGMSGQELTSMNLDKTQLLES 224

Query: 181 VLAKNYXXXXXXXXXXXXFAFIAFMMGQSLEAFMQWKALVSLLLSCSEAPLHTRTNLFVK 240
           VL+K Y            F+F+AF+MGQSLE+FMQWK++VSLLL C+ AP  TR+ LF K
Sbjct: 225 VLSKEYKDSEDLLLGELQFSFVAFLMGQSLESFMQWKSIVSLLLGCTSAPFQTRSQLFTK 284

Query: 241 FIRAIYYQLKHGFQHTQDNRSGEEMGNSLFLDEAWFSRDIFLYRLSKDFFAVILEATVVD 300
           FI+ IY+QLK+G Q      SG E G    LD++W + D FL+ L KDFFA++ E +VVD
Sbjct: 285 FIKVIYHQLKYGLQK---ENSGPETGIHALLDDSWLASDSFLHFLCKDFFALVEETSVVD 341

Query: 301 GDLLSWTRKLKSLLETTFGWDLDNNT-VNLI--DEDDEFAPVVVEMDGS 346
           GDLLSWTRK K LLE   GW+    + V+ I  +EDDE+APVV  +D S
Sbjct: 342 GDLLSWTRKFKELLENRLGWEFQKKSAVDGIYFEEDDEYAPVVEMLDES 390



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.321    0.136    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 7,356,120
Number of extensions: 283333
Number of successful extensions: 547
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 544
Number of HSP's successfully gapped: 1
Length of query: 346
Length of database: 11,106,569
Length adjustment: 99
Effective length of query: 247
Effective length of database: 8,392,385
Effective search space: 2072919095
Effective search space used: 2072919095
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 112 (47.8 bits)