BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os04g0118100 Os04g0118100|AK106028
(445 letters)
Database: tair10
27,416 sequences; 11,106,569 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AT1G08980.1 | chr1:2884455-2886430 FORWARD LENGTH=426 413 e-115
AT3G17970.1 | chr3:6148030-6151794 FORWARD LENGTH=590 323 1e-88
AT5G09420.1 | chr5:2928316-2931750 FORWARD LENGTH=604 295 3e-80
AT3G25660.1 | chr3:9339640-9342044 REVERSE LENGTH=538 64 2e-10
AT5G64440.1 | chr5:25766229-25770260 FORWARD LENGTH=608 57 2e-08
AT5G07360.1 | chr5:2326925-2330011 REVERSE LENGTH=660 54 2e-07
>AT1G08980.1 | chr1:2884455-2886430 FORWARD LENGTH=426
Length = 425
Score = 413 bits (1061), Expect = e-115, Method: Compositional matrix adjust.
Identities = 213/417 (51%), Positives = 271/417 (64%), Gaps = 6/417 (1%)
Query: 20 DYGAFMERFVXXXXXXXXXXX--HGLTFAIKDIFDIAGRVTGFGNPDWARTHAPAAATSP 77
D+GAF+E+ GLTFAIKDIFD+ GRVTGFGNPDW RTH+ A +T+P
Sbjct: 6 DFGAFIEKVTISPTSTSSSPPSLQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTAP 65
Query: 78 VVXXXXXXXXXXXXXXIMDEMAYSINGENTHYGTPTNPCXXXXXXXXXXXXXXXXXXXNL 137
VV IMDEMAYSINGEN HYGTP NP L
Sbjct: 66 VVSSLLEAGATALGITIMDEMAYSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAARL 125
Query: 138 VDFSLGTDTGGSVRVPAAYCGIFGLRPSHGLVSAENVIPMAQMFDTVGWFSRDLSTLSRV 197
VDFS+GTDTGGSVRVPA+YCGIFG RPSHG VS + PMAQ FDTVGWF+RD +TL RV
Sbjct: 126 VDFSIGTDTGGSVRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTVGWFARDTATLKRV 185
Query: 198 TKVLLPLPDDIVKQPTQVTIPMDCFQILGSLDDRTYQIINASVAKRFD-SQILDNRNLGD 256
VLL +P+Q+ I DCF++ D Q + SV K F + ++ NLG+
Sbjct: 186 GCVLLQQHHLNPIEPSQLIIADDCFKLCSVPHDLLVQPLVGSVEKSFGGNTVVKKVNLGE 245
Query: 257 FISDNVPSIGKFIT--DFSESELPSVPALSVISHVMRGLQRSQFKANHAEWVNTVKPNLG 314
+I NVPS+ F+T D + + +P+L +S MR LQR +FK NH W+++VKP G
Sbjct: 246 YIGQNVPSLKHFMTSDDVTTQQEFCIPSLMALSSSMRLLQRHEFKINHGAWISSVKPEFG 305
Query: 315 PGLRERILEAIASGDNESLEDFQAIRAEFKSALAALLKDHGILAIPTVPGPPPKVGMEAA 374
PG+ ERI EAI + D E ++ +++++E +AL+ LL + G+L IPTVPGPPP + A
Sbjct: 306 PGISERIEEAIRTSD-EKIDHCRSVKSELITALSTLLGEKGVLVIPTVPGPPPHLQANVA 364
Query: 375 PLENFRARAFSLLSIAGLSGFCQVSIPLGMRNGLPVSVSLVARHGADHFLLNVVEEL 431
LE+FR+RAFSLLSIAG+SGFCQVSIPLG+ LPVSVSLVA++G+D FLL++V+ L
Sbjct: 365 ALESFRSRAFSLLSIAGVSGFCQVSIPLGLHENLPVSVSLVAKYGSDGFLLSLVDSL 421
>AT3G17970.1 | chr3:6148030-6151794 FORWARD LENGTH=590
Length = 589
Score = 323 bits (828), Expect = 1e-88, Method: Compositional matrix adjust.
Identities = 177/423 (41%), Positives = 248/423 (58%), Gaps = 7/423 (1%)
Query: 18 RGDYGAFMERFVXXXXXXXXXXXH-----GLTFAIKDIFDIAGRVTGFGNPDWARTHAPA 72
R D+GAF+++ + GLTFA+ D+FDI G VTGFG+PDW RTH A
Sbjct: 31 REDFGAFIDKLMLLPPPQPAPPKAPHPLTGLTFAVSDVFDITGYVTGFGHPDWVRTHEAA 90
Query: 73 AATSPVVXXXXXXXXXXXXXXIMDEMAYSINGENTHYGTPTNPCXXXXXXXXXXXXXXXX 132
++TSPVV ++DE A+SI+GEN HY +PTNP
Sbjct: 91 SSTSPVVSTLVEGGATCVGKTVVDEFAFSISGENKHYDSPTNPAAPTRIPGGACSGAAVA 150
Query: 133 XXXNLVDFSLGTDTGGSVRVPAAYCGIFGLRPSHGLVSAENVIPMAQMFDTVGWFSRDLS 192
N VDF+LG DT G VRVPA YCG+ G + S+G +S +IP++ D+VGWF+RD +
Sbjct: 151 VATNAVDFALGIDTVGGVRVPAGYCGVLGFKSSYGAISNTGIIPVSSSLDSVGWFARDPN 210
Query: 193 TLSRVTKVLLPLPDDIVKQPTQVTIPMDCFQILGSLDDRTYQIINASVAKRFDSQILDNR 252
TL RV VLL LP + P Q+ + DCFQ+L DR Q++ S K F Q+L ++
Sbjct: 211 TLRRVGHVLLQLPFATQRNPRQIILADDCFQLLKIPVDRITQVVTKSAEKLFGRQLLKHQ 270
Query: 253 NLGDFISDNVPSIGKFITDFSESELPSVPALSVISHVMRGLQRSQFKANHAEWVNTVKPN 312
NL + VPS+ +F + + V ++++VM+ LQR +F NH +W+NTVKP
Sbjct: 271 NLETYFETKVPSLKEFARTKAIANT-KVSTSRLLANVMQLLQRHEFLQNHGDWINTVKPA 329
Query: 313 LGPGLRERILEAIASGDNESLEDFQAIRAEFKSALAALLKDHGILAIPTVPGPPPKVGME 372
+ P + ++ E NE E+ AIR E + A+ +LLKD GIL IPT+P PPK+G +
Sbjct: 330 IDPVILSQVCEN-PELTNEETENLNAIRNETRVAIGSLLKDDGILVIPTLPAVPPKLGSK 388
Query: 373 AAPLENFRARAFSLLSIAGLSGFCQVSIPLGMRNGLPVSVSLVARHGADHFLLNVVEELY 432
E+++ RA SLLSIA +SG CQV++PLG P+SVS + RHG D FLL+ V+ +Y
Sbjct: 389 EITSEDYQNRASSLLSIASISGCCQVTVPLGHHEKCPISVSFIGRHGGDRFLLDTVQTMY 448
Query: 433 QTL 435
+L
Sbjct: 449 PSL 451
>AT5G09420.1 | chr5:2928316-2931750 FORWARD LENGTH=604
Length = 603
Score = 295 bits (756), Expect = 3e-80, Method: Compositional matrix adjust.
Identities = 159/426 (37%), Positives = 227/426 (53%), Gaps = 8/426 (1%)
Query: 20 DYGAFMERF-----VXXXXXXXXXXXHGLTFAIKDIFDIAGRVTGFGNPDWARTHAPAAA 74
D+GAF++RF GLTF+I D FD+ +TGFG P W +TH A
Sbjct: 46 DFGAFLDRFELLPFPPPPPPAAKQSLSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAEK 105
Query: 75 TSPVVXXXXXXXXXXXXXXIMDEMAYSINGENTHYGTPTNPCXXXXXXXXXXXXXXXXXX 134
T+ VV IMDE+ + I GEN HYGTP NP
Sbjct: 106 TAVVVTTLLKNGATCVGKTIMDELGFGIIGENKHYGTPINPLMPDNVPGGCSSGSAVSVG 165
Query: 135 XNLVDFSLGTDTGGSVRVPAAYCGIFGLRPSHGLVSAENVIPMAQMFDTVGWFSRDLSTL 194
LVDFSLG DT G VRVPAA+CGI G RPS G VS+ V+P +Q +TVGWF+ D S L
Sbjct: 166 AELVDFSLGIDTTGGVRVPAAFCGILGFRPSQGTVSSVGVLPNSQSLETVGWFASDPSVL 225
Query: 195 SRVTKVLLPLPDDIVKQPTQVTIPMDCFQILGSLDDRTYQIINASVAKRFDSQILDNRNL 254
+V LL L ++ + D F++ ++ Q++ ++ + + N+
Sbjct: 226 CQVGHALLNLSAVTHRRQRSLIFADDLFELSDIPKQKSVQVVRKAIENLSGYKTPKHVNV 285
Query: 255 GDFISDNVPSIGKFITDFSESELPSVPALSVISHVMRGLQRSQFKANHAEWVNTVKPNLG 314
G +++ NVPS+ +F +S+ S L +S VM +QR +FK NH EW T K LG
Sbjct: 286 GQYVASNVPSLAEFCEQSGKSQ-NSASTLRALSSVMLAIQRHEFKTNHEEWWQTCKSFLG 344
Query: 315 PGLRERILEAIASGDNESLEDFQAIRAEFKSALAALLKDHGILAIPTVPGPPPKVGMEA- 373
P ++ A+ S NES++ ++ E ++ + +LLK+ GIL IPTV PPP++ +
Sbjct: 345 PRFSNDVVTALKS-KNESIKSLYRVKNEMRATIQSLLKEDGILVIPTVADPPPRLNTKRN 403
Query: 374 APLENFRARAFSLLSIAGLSGFCQVSIPLGMRNGLPVSVSLVARHGADHFLLNVVEELYQ 433
L F R ++L IA +SG CQV+IPLG P+SVSL+ +G D FLL+ ++Y
Sbjct: 404 KSLNEFLDRTYALSCIASMSGCCQVTIPLGEHGDRPISVSLLTYYGGDKFLLDTTLDVYA 463
Query: 434 TLIDEA 439
+L D+A
Sbjct: 464 SLQDQA 469
>AT3G25660.1 | chr3:9339640-9342044 REVERSE LENGTH=538
Length = 537
Score = 63.9 bits (154), Expect = 2e-10, Method: Compositional matrix adjust.
Identities = 84/329 (25%), Positives = 147/329 (44%), Gaps = 46/329 (13%)
Query: 141 SLGTDTGGSVRVPAAYCGIFGLRPSHGLVSAENVIPMAQMFDTVGWFSRDLSTLSRVTKV 200
SLG+DTGGSVR PA++CG+ GL+P++G VS ++ A D +G F ST++ +
Sbjct: 207 SLGSDTGGSVRQPASFCGVVGLKPTYGRVSRFGLMAYASSLDVIGCFG---STVADAGML 263
Query: 201 LLPLP--DDIVKQPTQVTIPMDCFQILG-------SLDDRTYQIINASVAKRFDSQILDN 251
L + D ++ +P Q L L+ II ++ DS +
Sbjct: 264 LHAISGYDRFDSTSSKQDVPEFQSQFLSVDHFESKPLNGVKVGIIRETLEDGVDSGV--- 320
Query: 252 RNLGDFISDNVPSIGKFITDFSESELPS----VPALSVISHVMRGLQRSQFKANH----- 302
R+ + ++ ++G +T+ S LPS +PA VI+ S++
Sbjct: 321 RSATQEAASHLEALGCILTEVS---LPSFSLGLPAYYVIASSESSSNLSRYDGVRYGNQV 377
Query: 303 -AEWVNTVK-----PNLGPGLRERIL---EAIASGDNES-LEDFQAIRAEFKSALAALLK 352
AE +N + G ++ RIL A+++G ++ + Q +R + A L+
Sbjct: 378 MAEELNKLYECSRGEGFGGEVKMRILMGTYALSAGYYDAYYKRAQQVRTLIRKDFKAALE 437
Query: 353 DHGILAIPTVPGPPPKVGMEA-APLENFRARAFSLLSI-AGLSGFCQVSIPLGMR----N 406
+ IL P P K+G + PL + A ++++ L+G + +P G+ +
Sbjct: 438 QNDILISPAAPSAAYKIGEKKDDPLAMY---AGDIMTVNVNLAGLPAMVLPCGLVEGGPS 494
Query: 407 GLPVSVSLVARHGADHFLLNVVEELYQTL 435
GLPV + ++ + LL V QTL
Sbjct: 495 GLPVGLQMIGAAFDEEKLLKVGHIFEQTL 523
>AT5G64440.1 | chr5:25766229-25770260 FORWARD LENGTH=608
Length = 607
Score = 57.0 bits (136), Expect = 2e-08, Method: Compositional matrix adjust.
Identities = 38/126 (30%), Positives = 48/126 (38%), Gaps = 1/126 (0%)
Query: 42 GLTFAIKDIFDIAGRVTGFGNPDWARTHAPAAATSPVVXXXXXXXXXXXXXXIMDEMAYS 101
G+ IKD D T G W S VV M E+
Sbjct: 199 GIFVTIKDDIDCLPHPTN-GGTTWLHEDRSVEKDSAVVSKLRSCGAILLGKANMHELGMG 257
Query: 102 INGENTHYGTPTNPCXXXXXXXXXXXXXXXXXXXNLVDFSLGTDTGGSVRVPAAYCGIFG 161
G N++YGT NP L +LGTD GGSVR+P+A CGI G
Sbjct: 258 TTGNNSNYGTTRNPHDPKRYTGGSSSGSAAIVAAGLCSAALGTDGGGSVRIPSALCGITG 317
Query: 162 LRPSHG 167
L+ ++G
Sbjct: 318 LKTTYG 323
>AT5G07360.1 | chr5:2326925-2330011 REVERSE LENGTH=660
Length = 659
Score = 53.5 bits (127), Expect = 2e-07, Method: Compositional matrix adjust.
Identities = 39/150 (26%), Positives = 62/150 (41%), Gaps = 7/150 (4%)
Query: 41 HGLTFAIKDIFDIAGRVTGFGNPDWARTHAPAAATSPVVXXXXXXXXXXXXXXIMDEMAY 100
HG+ + +KDI + G T +G+ + A V + MAY
Sbjct: 269 HGIPYGLKDIVAVPGYKTTWGSTSFKDQFLDIEAW--VYKRLKASGAVLVAKLVTGSMAY 326
Query: 101 SINGENTHYGTPT-NPCXXXXXXXXXXXXXXXXXXXNLVDFSLGTDTGGSVRVPAAYCGI 159
++ +G T NP +V F++G++T GS+ PAA CGI
Sbjct: 327 ----DDIWFGGRTRNPWNIEEFSTGSSAGPAASTSAGMVPFAIGSETAGSMTYPAARCGI 382
Query: 160 FGLRPSHGLVSAENVIPMAQMFDTVGWFSR 189
LRP+ G V V+ +++ D +G F R
Sbjct: 383 TALRPTFGSVGRTGVMSISESLDKLGPFCR 412
Database: tair10
Posted date: Dec 8, 2010 11:30 AM
Number of letters in database: 11,106,569
Number of sequences in database: 27,416
Lambda K H
0.320 0.137 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 8,862,460
Number of extensions: 338607
Number of successful extensions: 759
Number of sequences better than 1.0e-05: 6
Number of HSP's gapped: 746
Number of HSP's successfully gapped: 6
Length of query: 445
Length of database: 11,106,569
Length adjustment: 102
Effective length of query: 343
Effective length of database: 8,310,137
Effective search space: 2850376991
Effective search space used: 2850376991
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 113 (48.1 bits)