BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0854000 Os03g0854000|AK061374
         (188 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G02910.1  | chr3:649986-650549 FORWARD LENGTH=188              197   3e-51
AT5G46720.1  | chr5:18959183-18959710 FORWARD LENGTH=176          146   7e-36

>AT3G02910.1 | chr3:649986-650549 FORWARD LENGTH=188
          Length = 187

 Score =  197 bits (501), Expect = 3e-51,   Method: Compositional matrix adjust.
 Identities = 100/173 (57%), Positives = 120/173 (69%), Gaps = 13/173 (7%)

Query: 26  TLVFTYGTLKRGFSNHGLLQDLARDGDASFVGAATTAPRLPLVCGPYRVPFLLNLPGAGH 85
           TLVFTYGTLKRGFSNH L+QDL R GDASF G   T  + PLVCGPYRVPFLLN PG+G+
Sbjct: 12  TLVFTYGTLKRGFSNHVLMQDLIRSGDASFKGVYQTLDKYPLVCGPYRVPFLLNKPGSGY 71

Query: 86  RVSGELYAVTPRGLDRLDELEGVSRAHYERLPISV------------LLAEGAQVDAV-A 132
            V+GELYAV+PRGL RLDELEG+SR HY R PI +            L  E      V A
Sbjct: 72  HVNGELYAVSPRGLSRLDELEGISRGHYIRQPIRLAAAEEEEEEEGDLETEAPSSCVVEA 131

Query: 133 YYAHRGYADDLWARSGKKGYPEYSPAVADGYIRRKDRPQQLTFLEQIRVFVSS 185
           YYAH+ Y ++LW R+  + +  Y+   A GY++R DRPQ L+FL+ IR+FVSS
Sbjct: 132 YYAHKSYEEELWRRNRGRSFGAYTENEARGYVKRNDRPQHLSFLDHIRIFVSS 184



>AT5G46720.1 | chr5:18959183-18959710 FORWARD LENGTH=176
          Length = 175

 Score =  146 bits (368), Expect = 7e-36,   Method: Compositional matrix adjust.
 Identities = 75/169 (44%), Positives = 100/169 (59%), Gaps = 6/169 (3%)

Query: 25  RTLVFTYGTLKRGFSNHGLLQDLARDGDASFVGAATTAPRLPLVCGPYRVPFLLNLPGAG 84
           + L+F YGTLKR   NH LL+DL    DA ++G  TT  + PLV G Y +P+L+N  G+G
Sbjct: 6   KNLIFVYGTLKRNHRNHFLLEDLISTNDAVYIGQRTTRLQYPLVTGLYGIPYLINKSGSG 65

Query: 85  HRVSGELYAVTPRGLDRLDELEGVSRAHYERLP------ISVLLAEGAQVDAVAYYAHRG 138
            ++ GELY+V+ RGL RLDELEG+   HYERLP                V A AY+AH G
Sbjct: 66  QKIRGELYSVSKRGLVRLDELEGIKVNHYERLPIEVIEEDDEEEESNGVVLAEAYFAHFG 125

Query: 139 YADDLWARSGKKGYPEYSPAVADGYIRRKDRPQQLTFLEQIRVFVSSQS 187
           + + LW + GK G  E+       Y+R KDRP   + L++I  FVSS +
Sbjct: 126 FGERLWEKKGKCGMCEFGENDGVLYVRPKDRPMFSSVLDEIETFVSSSN 174



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.321    0.139    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 3,619,657
Number of extensions: 147373
Number of successful extensions: 291
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 289
Number of HSP's successfully gapped: 2
Length of query: 188
Length of database: 11,106,569
Length adjustment: 93
Effective length of query: 95
Effective length of database: 8,556,881
Effective search space: 812903695
Effective search space used: 812903695
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 108 (46.2 bits)