BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0851800 Os03g0851800|Y10253
         (313 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT5G48840.1  | chr5:19803823-19805041 REVERSE LENGTH=311          373   e-104

>AT5G48840.1 | chr5:19803823-19805041 REVERSE LENGTH=311
          Length = 310

 Score =  373 bits (957), Expect = e-104,   Method: Compositional matrix adjust.
 Identities = 188/311 (60%), Positives = 227/311 (72%), Gaps = 11/311 (3%)

Query: 6   EPEVIRDKXXXXXXXXXXXXEGKTVAVVPTMGYLHQGHLSLIXXXXXXXXXDPVAIVVTI 65
           EPEVIRDK            +GKT+ +VPTMGYLH+GHLSL+              VV+I
Sbjct: 2   EPEVIRDKDSMRKWSRAMRSQGKTIGLVPTMGYLHEGHLSLVRQSLALTD----VTVVSI 57

Query: 66  YVNPSQFAPSEDLATYPSDFAGDLRKLAS-TGVVDAVFNPPDLY-----VXXXXXXXXXX 119
           YVNP QF+P+EDL+TYPSDF+GDL KLA+ +G    VFNP +LY                
Sbjct: 58  YVNPGQFSPTEDLSTYPSDFSGDLTKLAALSGGKVVVFNPKNLYDYGGETKKINDGGGNG 117

Query: 120 XXXISCLEEAAGDGHETWVRVERLEKGMCGASRPVFFRGVATIVSKLFNIIEPDVAVFGK 179
              +SC+EE  G GHETW+RVERLEKG CG SRPVFFRGVATIV+KLFNI+EPDVA+FGK
Sbjct: 118 GRVVSCVEEG-GLGHETWIRVERLEKGFCGKSRPVFFRGVATIVTKLFNIVEPDVALFGK 176

Query: 180 KDYQQWRVICRMVRDLDFAIEIIGSEIVREADGLAMSSRNVHLSREEREKALSISRSLVD 239
           KDYQQWR+I RMVRDL+F IEI+GS+I RE DGLAMSSRNV LS EER++ALSISRSL  
Sbjct: 177 KDYQQWRIIQRMVRDLNFGIEIVGSDIAREKDGLAMSSRNVRLSDEERQRALSISRSLAM 236

Query: 240 ARTGALKGNTDCKQIKNKIVQTLTETGGQVDYVEIVEQESLVPVEQIDGPVVICVAAWFG 299
           A+    +G T+C ++K+ I+Q +  + G+VDYVEIV+QE+L  VE+I   VVICVAAWFG
Sbjct: 237 AKASVAEGKTNCAELKDMIIQQVVGSAGRVDYVEIVDQETLEGVEEIKSGVVICVAAWFG 296

Query: 300 KVRLIDNIEID 310
            VRLIDNIEI+
Sbjct: 297 TVRLIDNIEIN 307



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.319    0.137    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 5,956,449
Number of extensions: 219151
Number of successful extensions: 460
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 458
Number of HSP's successfully gapped: 1
Length of query: 313
Length of database: 11,106,569
Length adjustment: 98
Effective length of query: 215
Effective length of database: 8,419,801
Effective search space: 1810257215
Effective search space used: 1810257215
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 111 (47.4 bits)