BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0842700 Os03g0842700|AK070821
         (218 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G19770.1  | chr3:6866916-6869114 FORWARD LENGTH=521            226   6e-60
AT5G09320.1  | chr5:2889007-2892016 REVERSE LENGTH=713            215   2e-56

>AT3G19770.1 | chr3:6866916-6869114 FORWARD LENGTH=521
          Length = 520

 Score =  226 bits (576), Expect = 6e-60,   Method: Compositional matrix adjust.
 Identities = 104/200 (52%), Positives = 152/200 (76%), Gaps = 3/200 (1%)

Query: 1   MTKLFDRAFASSAEDVKSDMEISEKIGLLQHFVRPHHLDIPKLLHNEAAWLLAVKELQKI 60
           MTKLF R FAS+ E+V +D ++ +K+ L+Q F+ P +LDI     NE++WLLA KELQKI
Sbjct: 87  MTKLFTRVFASNTEEVIADEKLFQKMSLVQQFISPENLDIQPTFQNESSWLLAQKELQKI 146

Query: 61  NSFKSPREKLSCIMSCCQVINNLLLNVSMSNDRTLSGADDFLPILIYITIKANPPQLHSN 120
           N +K+PR+KL CI++CC+VINNLLLN S++++    GAD+FLP+LIY+TIKANPPQLHSN
Sbjct: 147 NMYKAPRDKLVCILNCCKVINNLLLNASIASNENAPGADEFLPVLIYVTIKANPPQLHSN 206

Query: 121 LKFIQLFRRETRLISEVEYYLTNLISAKMFIMNVNGHSLSMEESVFQTHMESARLGNHIS 180
           L +IQ +RRE++L+ E  Y+ TN++SA+ FI N++  S+S++E+ F+ +MESAR    IS
Sbjct: 207 LLYIQRYRRESKLVGEAAYFFTNILSAESFISNIDAKSISLDEAEFEKNMESARA--RIS 264

Query: 181 VASTNSSQ-GLGTSTPGQNE 199
              + + Q G G++ P ++E
Sbjct: 265 GLDSQTYQTGHGSAPPPRDE 284



>AT5G09320.1 | chr5:2889007-2892016 REVERSE LENGTH=713
          Length = 712

 Score =  215 bits (547), Expect = 2e-56,   Method: Compositional matrix adjust.
 Identities = 97/173 (56%), Positives = 133/173 (76%)

Query: 1   MTKLFDRAFASSAEDVKSDMEISEKIGLLQHFVRPHHLDIPKLLHNEAAWLLAVKELQKI 60
           MTKLF R FAS+ EDV SD ++ +KI L+Q F+ P +LDI     N+ +WLLA KELQKI
Sbjct: 84  MTKLFPRVFASNTEDVISDEKLFQKISLVQQFISPENLDIQPTFQNQTSWLLAQKELQKI 143

Query: 61  NSFKSPREKLSCIMSCCQVINNLLLNVSMSNDRTLSGADDFLPILIYITIKANPPQLHSN 120
           N + +PR+KL CI+ CC+VINNLLLN S+++++   GAD FLP+LIY+TIKANPPQ HSN
Sbjct: 144 NMYNAPRDKLMCILRCCKVINNLLLNASIASNQNEPGADQFLPVLIYVTIKANPPQFHSN 203

Query: 121 LKFIQLFRRETRLISEVEYYLTNLISAKMFIMNVNGHSLSMEESVFQTHMESA 173
           L +IQ +RR+++L+ E  Y  TN++SA+ FI N++  SLSM+E+ F+T M+SA
Sbjct: 204 LLYIQRYRRQSKLVGEAGYLFTNILSAESFISNIDAKSLSMDEADFETKMKSA 256



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.318    0.132    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,358,402
Number of extensions: 163424
Number of successful extensions: 471
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 471
Number of HSP's successfully gapped: 2
Length of query: 218
Length of database: 11,106,569
Length adjustment: 95
Effective length of query: 123
Effective length of database: 8,502,049
Effective search space: 1045752027
Effective search space used: 1045752027
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 109 (46.6 bits)