BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0839100 Os03g0839100|AK109847
         (511 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT3G24200.2  | chr3:8748095-8751166 REVERSE LENGTH=508            592   e-169

>AT3G24200.2 | chr3:8748095-8751166 REVERSE LENGTH=508
          Length = 507

 Score =  592 bits (1527), Expect = e-169,   Method: Compositional matrix adjust.
 Identities = 291/461 (63%), Positives = 351/461 (76%), Gaps = 8/461 (1%)

Query: 51  DIAIVGGGMVGLAVACALSNMPLTKHLRVGIIDSNPALKSRNYLKKDGVPDSRVSTITPA 110
           DIAIVGGGMVG+A+A +L++ PLTKHL V IID+NP L  RN ++K   PD RVST+TPA
Sbjct: 55  DIAIVGGGMVGIALAASLASKPLTKHLNVAIIDNNPLLGRRNIIEKGHQPDPRVSTVTPA 114

Query: 111 TISFFRDIGAWDHVQQQRHAFFGKMQVWDYTGLGYTRYSARDVGKEYLGCVVENKVLCNS 170
           TISF +DIGAW +++QQRHA+F KMQVWDYTGLGYTRY+A DV ++ LGCVVENKVL +S
Sbjct: 115 TISFLKDIGAWKYIEQQRHAYFDKMQVWDYTGLGYTRYNANDVHQDVLGCVVENKVLQSS 174

Query: 171 LLLRLQEENGDIEKMIYPTRLISLALPSKSRQAPTREAISSGYPPEELNRSNLVKLELSD 230
            L  +QE   D++K +YP RL ++ +   S      E  S+    +   R  L KLELSD
Sbjct: 175 QLSCVQES--DLQKTVYPARLNAMDMLPSSSLTGLGEVPST---TDLFMRGRLAKLELSD 229

Query: 231 GQTLYSKLVVGADGSKSNVRQTAGIKTTGWNYPQSAIICTVEHITENDCAWQRFLPSGPI 290
           G  +Y+KLVVGADGSKS VR+ AGIKTTGWNY Q+AIICTVEH  EN  AWQRFLP+GPI
Sbjct: 230 GNRVYAKLVVGADGSKSRVRELAGIKTTGWNYSQNAIICTVEHTAENYTAWQRFLPNGPI 289

Query: 291 ALLPIGDNYSNIVWTMSPEESLRHKSMSPEDFVKSVNNALDFGYGPHPHSGSLDYYMEKL 350
           ALLPIGD +SNIVWTM P+E+   K M+ +DF+K+VN+ALD GYGP+P + S    +  L
Sbjct: 290 ALLPIGDKFSNIVWTMDPKEASDRKLMNEDDFIKAVNDALDSGYGPNPETTSSRDSLSWL 349

Query: 351 FSDIGSTAASTKECFEVPPKAIGVVCERMAFPLSLKHSHDYISKRLALVGDAAHTVHPLA 410
               G    S KE FE PPK + +  ERM FPLSL+H+ DY+SKR+ALVGD+AHTVHPLA
Sbjct: 350 ---TGDATISAKERFETPPKVVKLSSERMMFPLSLRHAKDYVSKRVALVGDSAHTVHPLA 406

Query: 411 GQGVNLGFGDAAALAKVIAEGVSVGADFGDISLLKRYENNRKAANVAMAALLDGFQKMYS 470
           GQGVNLGF DA AL+K IAEG+++G D G+ +LLKRYE  RK AN+AM A+LDG QKMYS
Sbjct: 407 GQGVNLGFADACALSKAIAEGIALGTDIGEANLLKRYEAERKPANIAMMAVLDGIQKMYS 466

Query: 471 VDFGPLNVLRAAAFHGAQYISPLKKNIISYAMGDAKWPLFS 511
           +DFGPLN LRAAAFHGA YISPLKK IISYA G+   PLFS
Sbjct: 467 LDFGPLNALRAAAFHGAHYISPLKKRIISYASGEQSLPLFS 507



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.317    0.134    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 11,578,349
Number of extensions: 502727
Number of successful extensions: 1218
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 1218
Number of HSP's successfully gapped: 1
Length of query: 511
Length of database: 11,106,569
Length adjustment: 103
Effective length of query: 408
Effective length of database: 8,282,721
Effective search space: 3379350168
Effective search space used: 3379350168
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 114 (48.5 bits)