BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0836300 Os03g0836300|AK061174
         (212 letters)

Database: tair10 
           27,416 sequences; 11,106,569 total letters

Searching..................................................done


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AT2G35460.1  | chr2:14905788-14906504 FORWARD LENGTH=239           56   2e-08
AT5G06320.1  | chr5:1931016-1931711 REVERSE LENGTH=232             50   8e-07

>AT2G35460.1 | chr2:14905788-14906504 FORWARD LENGTH=239
          Length = 238

 Score = 55.8 bits (133), Expect = 2e-08,   Method: Compositional matrix adjust.
 Identities = 46/167 (27%), Positives = 70/167 (41%), Gaps = 21/167 (12%)

Query: 47  KATADDAVLLRLALSPGSPPSNSTVSYNATVTLSLRNPNLYRGISYDPVAVAFSFNGTRF 106
           K    +A L R    P S      + YN ++  S+RNPN   GI YD + V   +   RF
Sbjct: 82  KFQVTEADLTRFEFDPRS----HNLHYNISLNFSIRNPNQRLGIHYDQLEVRGYYGDQRF 137

Query: 107 DESATVPAFYHRPRKTATFHVTVGGAGKPVPKLTAAGVAAFRAENATGRFEVEVRLDTVM 166
             +A + +FY   + T      +   G+ +  L A G   FR +  +G + ++V+L   +
Sbjct: 138 -SAANMTSFYQGHKNTTVVGTELN--GQKLVLLGAGGRRDFREDRRSGVYRIDVKLRFKL 194

Query: 167 QYKAR---------KARCPLAVICPLQLQLVDPDVAATAFQRTKCTV 204
           ++K           K +C L V  PL     D       F  TKC V
Sbjct: 195 RFKFGFLNSWAVRPKIKCHLKV--PLSTSSSDERF---QFHPTKCHV 236



>AT5G06320.1 | chr5:1931016-1931711 REVERSE LENGTH=232
          Length = 231

 Score = 50.1 bits (118), Expect = 8e-07,   Method: Compositional matrix adjust.
 Identities = 43/189 (22%), Positives = 71/189 (37%), Gaps = 13/189 (6%)

Query: 19  CLIWTAAIAGVGGLIXXXXXXXXXXXPPKATADDAVLLRLALSPGSPPSNSTVSYNATVT 78
            LI  A + G+  LI             K    DA L    L P      + + YN  + 
Sbjct: 51  ILITIAVLLGIAALIIWLIFRPNAI---KFHVTDAKLTEFTLDP-----TNNLRYNLDLN 102

Query: 79  LSLRNPNLYRGISYDPVAVAFSFNGTRFDESATVPAFYHRPRKTATFHVTVGGAGKPVPK 138
            ++RNPN   G+ YD + V   +   RF  S  +  FY   + T    V     G+ +  
Sbjct: 103 FTIRNPNRRIGVYYDEIEVRGYYGDQRFGMSNNISKFYQGHKNTTV--VGTKLVGQQLVL 160

Query: 139 LTAAGVAAFRAENATGRFEVEVRLDTVMQYK---ARKARCPLAVICPLQLQLVDPDVAAT 195
           L          +  +  + ++ +L   +++K    +  R    + C L++ L     +  
Sbjct: 161 LDGGERKDLNEDVNSQIYRIDAKLRLKIRFKFGLIKSWRFKPKIKCDLKVPLTSNSTSGF 220

Query: 196 AFQRTKCTV 204
            FQ TKC V
Sbjct: 221 VFQPTKCDV 229



  Database: tair10
    Posted date:  Dec 8, 2010 11:30 AM
  Number of letters in database: 11,106,569
  Number of sequences in database:  27,416
  
Lambda     K      H
   0.321    0.134    0.426 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 27416
Number of Hits to DB: 4,334,168
Number of extensions: 154594
Number of successful extensions: 336
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 341
Number of HSP's successfully gapped: 2
Length of query: 212
Length of database: 11,106,569
Length adjustment: 94
Effective length of query: 118
Effective length of database: 8,529,465
Effective search space: 1006476870
Effective search space used: 1006476870
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 109 (46.6 bits)